四物汤对辐射血虚小鼠骨髓中促红细胞生成素和粒细胞集落刺激因子基因表达的影响
[The effect of Siwu Tang on EPO and G-CSF gene expression in bone marrow of irradiated blood deficiency mice].
作者信息
Guo Ping, Liang Qian-De, Hu Jian-Jiang, Wang Ji-Feng, Wang Sheng-Qi
机构信息
Institute of Radiation Medicine, Academy of Military Medical Sciences, Bejing 100850, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2005 Aug;30(15):1173-6.
OBJECTIVE
To observe the blood enriching function of Siwu Tang and its effect on Epo and G-CSF gene expressions in bone marrow of blood deficiency mice, and thus provide the basis for understanding the molecular mechanism of blood enriching function of Siwu Tang.
METHOD
The animal model of blood deficiency were established in the mice by using 3.5 Gy60Co gamma-ray. Peripheral blood cells were analyzed and CFU-GM, BFU-E, CFU-E and CFU-mix were counted in bone marrow colony cultured. Both Epo and G-CSF gene expressions in bone marrow were measured with RT-PCR.
RESULT
Siwu Tang significantly increased the number of peripheral blood cells and the amount of CFU-GM, BFU-E, CFU-E and CFU-mix in bone marrow and enhanced Epo and G-CSF gene expression in bone marrow in the mice with blood deficiency.
CONCLUSION
The promotion of Epo and G-CSF gene expressions in bone marrow may be one of the mechanisms underlying the blood enriching function of Siwu Tang decoction.
目的
观察四物汤的补血作用及其对血虚小鼠骨髓中促红细胞生成素（Epo）和粒细胞集落刺激因子（G-CSF）基因表达的影响，为揭示四物汤补血作用的分子机制提供依据。
方法
采用3.5 Gy 60Coγ射线照射建立小鼠血虚动物模型。分析外周血细胞，并对骨髓集落培养中的粒-巨噬细胞集落形成单位（CFU-GM）、爆式红系集落形成单位（BFU-E）、红系集落形成单位（CFU-E）和混合集落形成单位（CFU-mix）进行计数。采用逆转录聚合酶链反应（RT-PCR）检测骨髓中Epo和G-CSF基因的表达。
结果
四物汤可显著增加血虚小鼠外周血细胞数量以及骨髓中CFU-GM、BFU-E、CFU-E和CFU-mix的数量，并增强骨髓中Epo和G-CSF基因的表达。
结论
促进骨髓中Epo和G-CSF基因表达可能是四物汤水煎剂补血作用的机制之一。
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