Department of Radiology, Methodist Hospital Research Institute, Weill Cornell Medical College, Houston, TX 77030, USA.
Bioconjug Chem. 2011 Sep 21;22(9):1758-62. doi: 10.1021/bc200282t. Epub 2011 Aug 15.
New fluorogenic dyes were designed and synthesized based on Cu(I)-catalyzed 'click' reaction. Conjugating weakly fluorescent benzothiazole derivatives with an electron-deficient alkyne group at the 2-position with azide-containing molecules in aqueous solution form 'click-on' fluorescent adducts. Model reactions and cell culture experiment indicated that the developed 'click-on' dye could be applied to labeling various biomolecules, such as nucleic acids, proteins, and other molecules, in cells.
新的荧光染料是基于 Cu(I)-催化的“点击”反应设计和合成的。将弱荧光苯并噻唑衍生物与 2-位带有缺电子炔基的基团与含叠氮化物的分子在水溶液中连接，形成“点击”荧光加合物。模型反应和细胞培养实验表明，开发的“点击”染料可应用于标记细胞中的各种生物分子，如核酸、蛋白质和其他分子。
