Department of Civil and Environmental Engineering, The Pennsylvania State University, University Park, PA 16802, USA.
Bioresour Technol. 2011 Oct;102(19):8790-8. doi: 10.1016/j.biortech.2011.07.017. Epub 2011 Jul 21.
Anaerobic digester failure due to a pH drop may be overcome with the use of an acidotolerant methanogenic community. To test this, lab-scale reactors were inoculated from acidic bog sediments, a municipal sludge digester, or a combination of these inocula and challenged with glucose pulses without pH control. Only the bog reactor survived the first glucose shock, and the methanogen community was dominated by members of the acidic Fen Cluster. After restarting the digester and hybrid reactors, two subsequent glucose shocks were applied. Methanogenic communities converged in all reactors and were dominated by Methanosarcina and Methanobacteriaceae. The Fen Cluster was eventually nondetectable in bog and hybrid reactors, presumably due to periods of circumneutral pH with only intermittent periods of low pH following glucose shocks. Although the resultant communities required base addition, an increase in Methanosarcina numbers after glucose pulses resulted in decreased acetate and increased reactor pH and methane production.
由于 pH 值下降导致的厌氧消化器故障可以通过使用耐酸产甲烷群落来克服。为了验证这一点，使用实验室规模的反应器，从酸性沼泽沉积物、城市污泥消化器或这些接种物的混合物中接种，并在没有 pH 控制的情况下用葡萄糖脉冲进行挑战。只有沼泽反应器在第一次葡萄糖冲击中幸存下来，并且产甲烷菌群落主要由酸性 Fen 群成员组成。在重新启动消化器和混合反应器后，应用了两个后续的葡萄糖冲击。在所有反应器中，产甲烷菌群落都趋于一致，主要由 Methanosarcina 和 Methanobacteriaceae 组成。Fen 群最终在沼泽和混合反应器中无法检测到，推测是由于 pH 值呈中性，只有在葡萄糖冲击后间歇性地出现低 pH 值。尽管由此产生的群落需要添加碱，但葡萄糖脉冲后 Methanosarcina 数量的增加导致乙酸盐减少，反应器 pH 值和甲烷产量增加。
