Department of Chemical Engineering, Institute of Technology, Universidade de Santiago de Compostela, Constantino Candeira s/n, 15782, Santiago de Compostela, Galicia, Spain.
Appl Microbiol Biotechnol. 2018 Dec;102(23):10285-10297. doi: 10.1007/s00253-018-9383-9. Epub 2018 Oct 1.
Knowledge of connections between operational conditions, process stability, and microbial community dynamics is essential to enhance anaerobic digestion (AD) process efficiency and management. In this study, the detailed temporal effects of a sudden glycerol-based organic overloading on the AD microbial community and process imbalance were investigated in two replicate anaerobic digesters by a time-intensive sampling scheme. The microbial community time response to the overloading event was shorter than the shifts of reactor performance parameters. An increase in bacterial community dynamics and in the abundances of several microbial taxa, mainly within the Firmicutes, Tenericutes, and Chloroflexi phyla and Methanoculleus genera, could be detected prior to any shift on the reactor operational parameters. Reactor acidification already started within the first 24 h of the shock and headed the AD process to total inhibition in 72 h alongside with the largest shifts on microbiome, mostly the increase of Anaerosinus sp. and hydrogenotrophic methanogenic Archaea. In sum, this work proved that AD microbial community reacts very quickly to an organic overloading and some shifts occur prior to alterations on the performance parameters. The latter is very interesting as it can be used to improve AD process management protocols.
了解操作条件、过程稳定性和微生物群落动态之间的联系对于提高厌氧消化（AD）过程的效率和管理至关重要。在这项研究中，通过密集的采样方案，在两个重复的厌氧消化器中研究了甘油基有机过载对 AD 微生物群落和过程失衡的详细时间影响。微生物群落对过载事件的响应时间短于反应器性能参数的变化。在反应器操作参数发生任何变化之前，可以检测到细菌群落动态的增加和几个微生物类群的丰度增加，主要在厚壁菌门、无壁菌门和绿弯菌门以及产甲烷微球菌属内。在冲击的前 24 小时内，反应器酸化已经开始，并在 72 小时内导致 AD 过程完全抑制，同时微生物组发生最大变化，主要是 Anaerosinus sp. 和产氢甲烷古菌的增加。总之，这项工作证明 AD 微生物群落对有机过载反应非常迅速，并且在性能参数发生变化之前就已经发生了一些变化。后者非常有趣，因为它可用于改进 AD 过程管理协议。
