用于筛选磷霉素耐药性的选择性培养基。
Selective Culture Medium for Screening of Fosfomycin Resistance in .
机构信息
Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourggrid.8534.a, Fribourg, Switzerland.
INSERM European Unit (IAME), University of Fribourggrid.8534.a, Fribourg, Switzerland.
出版信息
J Clin Microbiol. 2022 Jan 19;60(1):e0206321. doi: 10.1128/JCM.02063-21. Epub 2021 Oct 20.
Abstract
A selective medium for screening fosfomycin (FOS)-resistant was developed. Performances of this medium were first evaluated by using cultures of a collection of 84 enterobacterial clinical strains (42 FOS susceptible and 42 FOS resistant). The SuperFOS medium showed excellent sensitivity and specificity of detection (100%) in those conditions. Then, by testing spiked stool and spiked urine specimens, it revealed excellent performances, with lower limits of identification ranging from 10 to 10 CFU/ml. This screening medium allows easy and accurate detection of FOS-resistant isolates regardless of their resistance mechanisms.
摘要
开发了一种用于筛选磷霉素（FOS）耐药的选择性培养基。该培养基的性能首先通过使用 84 株肠杆菌临床菌株（42 株 FOS 敏感和 42 株 FOS 耐药）的培养物进行评估。在这些条件下，SuperFOS 培养基显示出优异的检测灵敏度和特异性（100%）。然后，通过测试粪便和尿液标本，它显示出优异的性能，鉴定下限范围为 10 至 10 CFU/ml。该筛选培养基可轻松准确地检测 FOS 耐药分离株，而与它们的耐药机制无关。
相似文献
1
Selective Culture Medium for Screening of Fosfomycin Resistance in .用于筛选磷霉素耐药性的选择性培养基。
J Clin Microbiol. 2022 Jan 19;60(1):e0206321. doi: 10.1128/JCM.02063-21. Epub 2021 Oct 20.
2
[Activity of fosfomycin against extended-spectrum beta-lactamase producing Escherichia coli and Klebsiella pneumoniae].磷霉素对产超广谱β-内酰胺酶大肠埃希菌和肺炎克雷伯菌的活性
Enferm Infecc Microbiol Clin. 2006 Dec;24(10):613-6. doi: 10.1157/13095372.
3
Sensitivity of extended spectrum of β-lactamase producing Escherichia coli and Klebsiella species to Fosfomycin.产超广谱β-内酰胺酶大肠埃希菌和克雷伯菌属对磷霉素的敏感性
J Pak Med Assoc. 2020 Jul;70(7):1187-1192. doi: 10.5455/JPMA.32169.
4
Molecular Epidemiology of Plasmid-Mediated Fosfomycin Resistance Gene Determinants in Klebsiella pneumoniae Carbapenemase-Producing Klebsiella pneumoniae Isolates in China.中国产肺炎克雷伯菌碳青霉烯酶肺炎克雷伯菌分离株中质粒介导磷霉素耐药基因决定子的分子流行病学
Microb Drug Resist. 2019 Mar;25(2):251-257. doi: 10.1089/mdr.2018.0137. Epub 2018 Aug 16.
5
First Detection of Isolate Co-Harboring Fosfomycin Resistance Gene and ctx-m Among Gram Negative Urine Isolates in a Turkish Hospital.土耳其某医院革兰氏阴性尿液分离株中首次同时检测到磷霉素耐药基因 和 ctx-m。
Microb Drug Resist. 2022 Mar;28(3):317-321. doi: 10.1089/mdr.2021.0114. Epub 2021 Dec 1.
6
Antimicrobial susceptibilities of extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae in health care-associated urinary tract infection: focus on susceptibility to fosfomycin.医疗保健相关尿路感染中产超广谱β-内酰胺酶大肠埃希菌和肺炎克雷伯菌的抗菌药敏性：聚焦对磷霉素的敏感性
Int Urol Nephrol. 2015 Jul;47(7):1059-66. doi: 10.1007/s11255-015-1018-9. Epub 2015 May 31.
7
Prevalence and mechanisms of fosfomycin resistance among KPC-producing Klebsiella pneumoniae clinical isolates in China.中国产 KPC 肺炎克雷伯菌临床分离株中磷霉素耐药的流行情况及机制。
Int J Antimicrob Agents. 2021 Jan;57(1):106226. doi: 10.1016/j.ijantimicag.2020.106226. Epub 2020 Nov 16.
8
[Mechanisms of fosfomycin resistance of extended-spectrum β-lactamases-producing and ].[产超广谱β-内酰胺酶菌株对磷霉素的耐药机制及] （原文句子不完整，翻译可能不太准确，仅供参考）
Zhonghua Yi Xue Za Zhi. 2018 Jan 9;98(2):122-126. doi: 10.3760/cma.j.issn.0376-2491.2018.02.010.
9
Fosfomycin resistance mechanisms in : an increasing threat.磷霉素耐药机制：日益严峻的威胁。
Front Cell Infect Microbiol. 2023 Jul 4;13:1178547. doi: 10.3389/fcimb.2023.1178547. eCollection 2023.
10
fosA3 overexpression with transporter mutations mediates high-level of fosfomycin resistance and silence of fosA3 in fosfomycin-susceptible Klebsiella pneumoniae producing carbapenemase clinical isolates.携带转运基因突变的 fosA3 过度表达导致高产水平的磷霉素耐药性，并使产碳青霉烯酶的敏感型肺炎克雷伯菌临床分离株中的 fosA3 沉默。
PLoS One. 2020 Aug 28;15(8):e0237474. doi: 10.1371/journal.pone.0237474. eCollection 2020.
引用本文的文献
1
Emergence of plasmid-mediated fosfomycin resistance among harboring , (X4), and genes in wild birds.野生鸟类中携带、（X4）和基因的质粒介导磷霉素耐药性的出现。
mSystems. 2025 Apr 22;10(4):e0167324. doi: 10.1128/msystems.01673-24. Epub 2025 Mar 13.
2
The Probiotic Potential and Metabolite Characterization of Bioprotective and for Applications in Animal Production.生物保护剂在动物生产中的益生菌潜力及代谢产物特性与应用
Animals (Basel). 2024 Jan 25;14(3):388. doi: 10.3390/ani14030388.
3
Fosfomycin resistance mechanisms in : an increasing threat.磷霉素耐药机制：日益严峻的威胁。
Front Cell Infect Microbiol. 2023 Jul 4;13:1178547. doi: 10.3389/fcimb.2023.1178547. eCollection 2023.
4
A Selective Culture Medium for Screening Cefiderocol Resistance in , Pseudomonas aeruginosa, and Acinetobacter baumannii.用于筛选铜绿假单胞菌和鲍曼不动杆菌中头孢地尔罗耐药性的选择性培养基。
J Clin Microbiol. 2023 Jul 20;61(7):e0188322. doi: 10.1128/jcm.01883-22. Epub 2023 Jun 20.
本文引用的文献
1
Mobile fosfomycin resistance genes in Enterobacteriaceae-An increasing threat.肠杆菌科中的移动磷霉素耐药基因——日益严重的威胁。
Microbiologyopen. 2020 Dec;9(12):e1135. doi: 10.1002/mbo3.1135. Epub 2020 Oct 30.
2
A Selective Culture Medium for Screening Ceftazidime-Avibactam Resistance in and Pseudomonas aeruginosa.一种用于筛选肺炎克雷伯菌和铜绿假单胞菌对头孢他啶-阿维巴坦耐药性的选择性培养基。
J Clin Microbiol. 2020 Aug 24;58(9). doi: 10.1128/JCM.00965-20.
3
Identification of , a Novel Plasmid-Mediated Fosfomycin Resistance Gene of Origin, in .从[具体来源]中鉴定出一种源自[具体来源]的新型质粒介导的磷霉素抗性基因。
Infect Drug Resist. 2020 May 1;13:1273-1279. doi: 10.2147/IDR.S251360. eCollection 2020.
4
Characterisation of plasmids harbouring extended-spectrum cephalosporin resistance genes in Escherichia coli from French rivers.法国河流中携带超广谱头孢菌素耐药基因的大肠杆菌质粒的特征。
Vet Microbiol. 2020 Apr;243:108619. doi: 10.1016/j.vetmic.2020.108619. Epub 2020 Feb 28.
5
IS-Mediated Transposition Leads to Fosfomycin and Broad-Spectrum Cephalosporin Resistance in Klebsiella pneumoniae.IS 介导的转座导致肺炎克雷伯菌对磷霉素和广谱头孢菌素的耐药性。
Antimicrob Agents Chemother. 2020 Apr 21;64(5). doi: 10.1128/AAC.00150-20.
6
Escherichia coli resistant to fosfomycin from urinary tract infections: Detection of the fosA3 gene in Spain.尿路感染中产黏菌素耐药大肠埃希菌的检测：在西班牙发现 fosA3 基因。
J Glob Antimicrob Resist. 2020 Jun;21:414-416. doi: 10.1016/j.jgar.2020.01.023. Epub 2020 Feb 12.
7
Characterization of FosL1, a Plasmid-Encoded Fosfomycin Resistance Protein Identified in Escherichia coli.FosL1的特性研究，FosL1是在大肠杆菌中鉴定出的一种质粒编码的磷霉素抗性蛋白。
Antimicrob Agents Chemother. 2020 Mar 24;64(4). doi: 10.1128/AAC.02042-19.
8
In vivo acquisition of fosfomycin resistance in Escherichia coli by fosA transmission from commensal flora.通过共生菌群的fosA基因传递，大肠杆菌在体内获得对磷霉素的耐药性。
J Antimicrob Chemother. 2019 Dec 1;74(12):3630-3632. doi: 10.1093/jac/dkz380.
9
Identification of FosA8, a Plasmid-Encoded Fosfomycin Resistance Determinant from Escherichia coli, and Its Origin in Leclercia adecarboxylata.鉴定大肠埃希菌中一种由质粒编码的磷霉素耐药决定因子 FosA8 及其在莱氏柠檬酸杆菌中的起源。
Antimicrob Agents Chemother. 2019 Oct 22;63(11). doi: 10.1128/AAC.01403-19. Print 2019 Nov.
10
Prevalence of fosfomycin resistance among ESBL-producing Escherichia coli isolates in the community, Switzerland.社区中产 ESBL 大肠杆菌分离株中磷霉素耐药的流行情况。瑞士。
Eur J Clin Microbiol Infect Dis. 2019 May;38(5):945-949. doi: 10.1007/s10096-019-03531-0. Epub 2019 Mar 15.
Zotero 插件