Toda Toshihiko, Sunagawa Tadahiro, Kanda Tomomasa, Tagashira Motoyuki, Shirasawa Takuji, Shimizu Takahiko
Molecular Gerontology, Tokyo Metropolitan Institute of Gerontology, 35-2 Sakae-cho, Itabashi-ku, Tokyo 173-0015, Japan.
Biochem Res Int. 2011;2011:784698. doi: 10.1155/2011/784698. Epub 2011 Aug 2.
Procyanidins (PCs) are major components of the apple polyphenols (APs). We previously reported that treatment with PC extended the mean lifespan of Caenorhabditis elegans (Sunagawa et al., 2011). In order to estimate the neuroprotective effects of PC, we investigated the antiaggregative activity of PC on amyloid β-protein (Aβ) aggregation, which is a pathological hallmark of Alzheimer's disease. We herein report that PC significantly suppressed Aβ42 aggregation and dissociated Aβ42 aggregates in a dose-dependent manner, indicating that PC is a potent suppressor of Aβ aggregation. Furthermore, PC significantly inhibited Aβ42 neurotoxicity and stimulated proliferation in PC-12 cells. These results suggested that the PC and AP acted as neuroprotective factors against toxic Aβ aggregates.
原花青素（PCs）是苹果多酚（APs）的主要成分。我们之前报道过，用PC处理可延长秀丽隐杆线虫的平均寿命（砂川等人，2011年）。为了评估PC的神经保护作用，我们研究了PC对淀粉样β蛋白（Aβ）聚集的抗聚集活性，Aβ聚集是阿尔茨海默病的病理标志。我们在此报告，PC以剂量依赖的方式显著抑制Aβ42聚集并使Aβ42聚集体解离，表明PC是Aβ聚集的有效抑制剂。此外，PC显著抑制Aβ42的神经毒性并刺激PC-12细胞增殖。这些结果表明，PC和AP可作为针对有毒Aβ聚集体的神经保护因子。
