Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Int J Syst Evol Microbiol. 2012 Jun;62(Pt 6):1354-1358. doi: 10.1099/ijs.0.033340-0. Epub 2011 Aug 5.
A Gram-stain-negative, aerobic, non-flagellated, non-spore-forming, motile (by gliding) bacterial strain, designated M-M6(T), was isolated from marine sand of Geoje island, Korea. Strain M-M6(T) grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain M-M6(T) fell within the clade comprising Cellulophaga species, forming a coherent cluster with Cellulophaga lytica ATCC 23178(T) and Cellulophaga fucicola NN015860(T), with which it shared 16S rRNA gene sequence similarities of 98.1 and 98.2 %, respectively. Sequence similarities between strain M-M6(T) and the type strains of other recognized Cellulophaga species were in the range 92.4-93.8 %. Strain M-M6(T) contained MK-6 as the predominant menaquinone and iso-C(15:0), iso-C(15:1) G, iso-C(17:0) 3-OH, and C(16:1)ω7c and/or iso-C(15:0) 2-OH as the major fatty acids. The major polar lipids detected in strain M-M6(T) and the type strains of C. lytica and C. fucicola were two unidentified lipids, one unidentified aminolipid and one unidentified aminophospholipid. The DNA G+C content of strain M-M6(T) was 35.4 mol%. Levels of DNA-DNA relatedness between strain M-M6(T) and C. lytica JCM 8516(T) and C. fucicola JCM 21778(T) were 33 and 35 %, respectively. Differential phenotypic properties and phylogenetic and genetic distinctiveness distinguished strain M-M6(T) from all recognized Cellulophaga species. On the basis of the data presented, strain M-M6(T) is considered to represent a novel species of the genus Cellulophaga, for which the name Cellulophaga geojensis sp. nov. is proposed. The type strain is M-M6(T) ( = KCTC 23498(T) = CCUG 60801(T)).
一株革兰氏阴性、需氧、不产鞭毛、不产芽孢、可滑行运动的细菌菌株，被命名为 M-M6(T)，从韩国巨济岛的海洋沙中分离得到。菌株 M-M6(T) 在 25°C、pH7.0-8.0 和 2%（w/v）NaCl 的存在下生长最佳。基于 16S rRNA 基因序列的系统发育分析表明，菌株 M-M6(T) 属于纤维弧菌属，与纤维弧菌属 Lytica ATCC 23178(T) 和纤维弧菌属 Fucicola NN015860(T) 形成一个连贯的聚类，与它们的 16S rRNA 基因序列相似度分别为 98.1%和 98.2%。菌株 M-M6(T) 与其他公认的纤维弧菌属的模式菌株之间的序列相似度在 92.4-93.8%之间。菌株 M-M6(T) 含有 MK-6 作为主要的menaquinone 和 iso-C(15:0)、iso-C(15:1) G、iso-C(17:0) 3-OH 和 C(16:1)ω7c 和/或 iso-C(15:0) 2-OH 作为主要脂肪酸。在菌株 M-M6(T) 和纤维弧菌属 Lytica 和纤维弧菌属 Fucicola 的模式菌株中检测到的主要极性脂质是两种未鉴定的脂质、一种未鉴定的胺脂和一种未鉴定的胺磷脂。菌株 M-M6(T) 的 DNA G+C 含量为 35.4mol%。菌株 M-M6(T) 与纤维弧菌属 Lytica JCM 8516(T) 和纤维弧菌属 Fucicola JCM 21778(T) 之间的 DNA-DNA 同源性水平分别为 33%和 35%。菌株 M-M6(T) 在表型特征、系统发育和遗传上与所有已识别的纤维弧菌属物种都有明显的区别。基于提供的数据，菌株 M-M6(T) 被认为代表了纤维弧菌属的一个新种，因此提议将其命名为 Geojensis 属的 Cellulophaga geojensis sp. nov.。该模式菌株为 M-M6(T)（=KCTC 23498(T)=CCUG 60801(T)）。
