Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Feb;63(Pt 2):685-690. doi: 10.1099/ijs.0.040741-0. Epub 2012 Apr 27.
A Gram-staining-negative, non-spore-forming, aerobic, non-flagellated, non-gliding, rod-shaped bacterium, designated strain T-y7(T), was isolated from seawater collected in an oyster farm in the South Sea, South Korea, and subjected to a polyphasic study. Strain T-y7(T) grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2 % (w/v) NaCl. In phylogenetic analyses based on 16S rRNA gene sequences, strain T-y7(T) fell within a clade comprising Bizionia species. It formed a coherent cluster with the type strains of Bizionia algoritergicola, B. argentinensis, B. echini and B. myxarmorum, with which it exhibited 16S rRNA gene sequence similarities of 97.4-98.9 % and mean DNA-DNA relatedness values of 11-27 %. Strain T-y7(T) had MK-6 as its predominant menaquinone and iso-C(15 : 1) G, summed feature 3 (C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH), iso-C(15 : 0) and iso-C(17 : 0) 3-OH as its major fatty acids. The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and one unidentified lipid. The genomic DNA G+C content was 37.1 mol%. Its phylogenetic and genetic distinctiveness and differential phenotypic properties revealed that strain T-y7(T) does not belong to any established Bizionia species. On the basis of the data presented, strain T-y7(T) is considered to represent a novel species of the genus Bizionia, for which the name Bizionia hallyeonensis sp. nov. is proposed. The type strain is T-y7(T) ( = KCTC 23881(T) = CCUG 62110(T)).
一株革兰氏阴性、非孢子形成、需氧、无鞭毛、非滑行、杆状细菌，被命名为 T-y7(T) 菌株，从韩国南海牡蛎养殖场采集的海水中分离得到，并进行了多相研究。T-y7(T) 菌株在 25°C、pH 值 7.0-8.0 和 2%（w/v）NaCl 的条件下生长最佳。在基于 16S rRNA 基因序列的系统发育分析中，T-y7(T) 菌株属于 Bizionia 种。它与 Bizionia algoritergicola、B. argentinensis、B. echini 和 B. myxarmorum 的模式菌株形成一个连贯的聚类，与它们的 16S rRNA 基因序列相似度为 97.4-98.9%，平均 DNA-DNA 同源性值为 11-27%。T-y7(T) 菌株的主要类异戊二烯醌为 MK-6，主要脂肪酸为 iso-C(15：1) G、总和特征 3（C(16：1)ω7c 和/或 iso-C(15：0) 2-OH）、iso-C(15：0) 和 iso-C(17：0) 3-OH。主要极性脂为磷脂酰乙醇胺、一种未鉴定的氨基脂和一种未鉴定的脂。基因组 DNA G+C 含量为 37.1mol%。其系统发育和遗传独特性以及表型差异特征表明，T-y7(T) 菌株不属于任何已建立的 Bizionia 种。基于所提供的数据，T-y7(T) 菌株被认为代表 Bizionia 属的一个新种，因此提议将其命名为 Bizionia hallyeonensis sp. nov.。模式菌株为 T-y7(T)（=KCTC 23881(T)=CCUG 62110(T)）。
