Institute for Innovation, Ajinomoto Co., Inc., Kawasaki, Japan.
Ann Nutr Metab. 2011;58(3):250-8. doi: 10.1159/000330444. Epub 2011 Aug 9.
Grape seed extracts (GSE) are known to present health benefits such as antioxidative and anti-obesity effects in animal models. The purpose of this research is to determine whether the specially manufactured GSE, catechin-rich GSE (CGSE), can protect against obesity induced by a high-fat diet (HFD) and to address the mechanism underlying this effect.
The componential analysis of CGSE was performed using liquid chromatography/mass spectrometry. Oxygen consumption and the respiratory quotient were determined using 500 mg/kg CGSE administered orally for 3 days in 14- to 15-week-old male C57BL/6J mice. Nine-week-old male C57BL/6J mice were supplemented with 0.5 or 1% CGSE in a HFD for 12 weeks, and their body weight and food intake were monitored. Blood and tissue samples were collected and analyzed.
The main polyphenol components of CGSE were catechin and epicatechin. CGSE supplementation in the HFD-induced obesity model chronically suppressed the increase in body weight and the weight of fat pads. Furthermore, CGSE improved metabolic parameter abnormalities and upregulated the fatty acid oxidation-related genes in the liver.
These findings suggest that CGSE contains monomeric catechins in high concentrations and ameliorates HFD-induced obesity in C57BL/6J mice.
葡萄籽提取物（GSE）在动物模型中具有抗氧化和抗肥胖等健康益处。本研究旨在确定特制的 GSE、儿茶素丰富的 GSE（CGSE）是否可以预防高脂肪饮食（HFD）引起的肥胖，并探讨其作用机制。
采用液相色谱/质谱法对 CGSE 的成分进行分析。用 14-15 周龄雄性 C57BL/6J 小鼠口服 500mg/kg CGSE 连续 3 天，测定其耗氧量和呼吸商。9 周龄雄性 C57BL/6J 小鼠用 0.5%或 1% CGSE 补充 HFD 12 周，监测其体重和食物摄入量。采集血液和组织样本并进行分析。
CGSE 的主要多酚成分是儿茶素和表儿茶素。CGSE 补充 HFD 诱导的肥胖模型可长期抑制体重和脂肪垫重量的增加。此外，CGSE 改善了代谢参数异常，并上调了肝脏中与脂肪酸氧化相关的基因。
这些发现表明 CGSE 含有高浓度的单体儿茶素，可改善 C57BL/6J 小鼠的 HFD 诱导肥胖。
