Division of Advanced Prosthetic Dentistry, Tohoku University Graduate School of Dentistry, 4-1 Seiryo-machi, Aoba-ku, Sendai, 980-8575, Japan.
Department of Orthopaedics Surgery, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan.
Sci Rep. 2020 Jun 1;10(1):8812. doi: 10.1038/s41598-020-65403-4.
The purpose of the present study was to confirm if proanthocyanidin-rich grape seed extract (GSE) had the ability to improve bone health such as bone loss, bone healing, and implant osseointegration (defined as the direct connection between bone tissue and an implant) in ovariectomized (OVX) animals. We demonstrated that daily oral administration of GSE prevented bone loss in the lumbar vertebrae and femur in OVX mice. In addition, osteoclastogenesis in the lumbar spine bone of OVX mice, as assessed by histological and histomorphometric analyses, was accelerated but GSE prevented this dynamization, suggesting that GSE could counteract OVX-induced accelerated osteoclastogenic activity. In rats, OVX clearly impaired the healing of defects created on the calvaria, and GSE overcame this OVX-impaired healing. In the same way, osseointegration of a tibial implant in rats was retarded by OVX, and GSE counteracted the OVX-induced poor osseointegration, likely promoting bone healing by preventing imbalanced bone turnover. These results suggest that orally administered GSE improved implant osseointegration by mitigating the impaired bone health induced by OVX as a model of estrogen deficiency.
本研究旨在确认富含原花青素的葡萄籽提取物 (GSE) 是否具有改善骨质健康的能力，如去卵巢 (OVX) 动物的骨丢失、骨愈合和种植体骨整合 (定义为骨组织与植入物之间的直接连接)。我们证明，GSE 的每日口服给药可预防 OVX 小鼠腰椎和股骨的骨丢失。此外，通过组织学和组织形态计量学分析评估，OVX 小鼠腰椎骨中的破骨细胞生成加速，但 GSE 阻止了这种动态变化，表明 GSE 可以对抗 OVX 诱导的破骨细胞生成活性加速。在大鼠中，OVX 明显损害了颅骨上形成的缺损的愈合，而 GSE 克服了这种 OVX 损害的愈合。同样，OVX 延迟了大鼠胫骨植入物的骨整合，而 GSE 拮抗了 OVX 诱导的不良骨整合，可能通过防止骨转换失衡来促进骨愈合。这些结果表明，口服 GSE 通过减轻 OVX 引起的骨健康受损，改善了种植体骨整合，OVX 作为雌激素缺乏的模型。
