Guimarães Gustavo N, Pires-De-Campos Maria S M, Leonardi Gislaine R, Dib-Giusti Helena H K, Polacow Maria L O
Faculdade de Ciências da Saúde, Universidade Metodista de Piracicaba, Piracicaba (UNIMEP), SP, Brasil.
Rev Bras Fisioter. 2011 May-Jun;15(3):227-32.
To analyze the effect of ultrasound (US), dexpanthenol (d-P) and a combination of these treatments (US+d-P) on collagen fiber organization in tegumentary lesions in rats by birefringence analysis.
Wistar rats (50) were anesthetized (Thionembutal - Sodic = 50 mg/Kg), 1 cm² of dorsal region skin was removed, and the animals were divided into five groups: control (C), gel (G), US (3 MHz, 0.1 W/cm2, 1 minute, continuous), d-P (10%) and US+d-P. After daily treatment for 7 and 14 days, 6 µm thick sections of lesioned areas were stained in picrosirius and measurements of the collagen birefringent area (µm²) were obtained using polarized light microscopy (Zeiss Axiolab-ZEISS- Germany) with histological image analysis software (KS 400 2.0 - Kontrol Eletronics, Munique, Germany). The means were compared by ANOVA followed by the Tukey test (p<0.05).
The US+d-P group showed a significantly greater (p ≤ 0.001) birefringent area (1586.43±162.14) than the other experimental groups: C (139.36±35.35), US (317.55±129.9) and d-P (192.41±3657) by the 7th day of treatment, indicating acceleration of the wound healing process. By the 14th day of treatment, the US+d-P, US and d-P groups presented greater birefringence than the control group, but did not differ from each other.
The combination of treatments (US+d-P) accelerated collagen fiber synthesis and organization in the early stages of cutaneous repair.
通过双折射分析，研究超声（US）、泛醇（d - P）及二者联合治疗（US + d - P）对大鼠皮肤损伤处胶原纤维组织的影响。
将50只Wistar大鼠麻醉（硫喷妥钠 = 50 mg/Kg），切除1 cm²背部皮肤，动物被分为五组：对照组（C）、凝胶组（G）、超声组（3 MHz，0.1 W/cm²，1分钟连续波）、泛醇组（10%）和超声 + 泛醇组。每日治疗7天和14天后，取损伤区域6 µm厚切片进行天狼星红染色，使用带有组织学图像分析软件（KS 400 2.0 - Kontrol Eletronics，德国慕尼黑）的偏光显微镜（蔡司AxioLab - 德国蔡司）测量胶原双折射面积（µm²）。采用方差分析及Tukey检验比较均值（p < 0.05）。
治疗第7天，超声 + 泛醇组的双折射面积（1586.43±16².14）显著大于其他实验组：对照组（139.36±35.35）、超声组（317.55±129.9）和泛醇组（192.41±36.57），表明伤口愈合过程加速。治疗第14天，超声 + 泛醇组、超声组和泛醇组的双折射均大于对照组，但彼此之间无差异。
联合治疗（超声 + 泛醇）在皮肤修复早期加速了胶原纤维的合成与组织化。
