Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Mansfield Road, Oxford OX1 3TA, UK.
Chem Commun (Camb). 2011 Sep 28;47(36):10010-2. doi: 10.1039/c1cc13524d. Epub 2011 Aug 11.
A strategy for the site-specific attachment of 2-deoxy-2-fluorosugars to cysteine and dehydroalanine tagged proteins is reported. When combined with thionation of fluorosugars, such as the widely available (18)F probe 2-deoxy-2-[(18)F]fluoroglucose ([(18)F]FDG), this methodology allows fast and direct access to site-specific [(18)F]FDG-labelled proteins.
报道了一种将 2-脱氧-2-氟糖定点连接到半胱氨酸和脱氢丙氨酸标记蛋白上的策略。当与氟糖的硫代反应（如广泛可用的（18）F 探针 2-脱氧-2-[(18)F]氟葡萄糖([(18)F]FDG)结合使用时，该方法允许快速直接获得定点[(18)F]FDG 标记蛋白。
