Faculty of Traditional Uighur Medicine, Xinjiang Medical University, Urumqi 830011, China.
Evid Based Complement Alternat Med. 2012;2012:863101. doi: 10.1155/2012/863101. Epub 2011 Aug 7.
Abnormal Savda Munziq (ASMq) is a herbal preparation used in Traditional Uighur Medicine for the treatment and prevention of diabetes, cardiovascular diseases, chronic asthma and cancer. The recommended dose of this decoction for cancer patients is 500 mL administered orally three times a day. Our approach aimed at reducing the high amount of fluid intake required by fractionation of ASMq guided by the antiproliferative activity on HL-60 cells. The fractionation of ASMq resulted in the preparation of an active extract, Extr-4. Using solid phase extraction, Extr-4 was further fractionated into five fractions (SPE-0, SPE-20, SPE-40, SPE-60 and SPE-80), with SPE-40 showing the strongest antiproliferative activity. Caffeic acid, rutin, isoquercitrin, isorhamnetin 3-O-rutinoside, apigenin 7-O-glucoside, rosmarinic acid, luteolin and formononetin were identified in Extr-4 and fractions thereof by means of TLC, HPLC-DAD and LC-MS. SPE-40 contained the main compounds responsible for the antiproliferative activity on HL-60 cells. Thus, a phenolic fraction with high antiproliferative activity on HL-60 cells was obtained from ASMq through the bioassay-guided fractionation process. This could provide a better pharmaceutical formulation that minimizes the administration inconveniencies of a high volume (1.5 L per day) of ASMq decoction for cancer patients.
异常沙枣（ASMq）是一种草药制剂，用于维吾尔传统医学治疗和预防糖尿病、心血管疾病、慢性哮喘和癌症。该汤剂推荐剂量为 500 毫升，癌症患者每天口服三次。我们的方法旨在减少因 HL-60 细胞的增殖活性指导下的 ASMq 分段所需的大量液体摄入。ASMq 的分段导致了活性提取物 Extr-4 的制备。使用固相萃取，Extr-4 进一步分为五个部分（SPE-0、SPE-20、SPE-40、SPE-60 和 SPE-80），其中 SPE-40 显示出最强的增殖活性。通过 TLC、HPLC-DAD 和 LC-MS，在 Extr-4 和其部分中鉴定出咖啡酸、芦丁、异槲皮苷、异鼠李素 3-O-芸香糖苷、芹菜素 7-O-葡萄糖苷、迷迭香酸、木犀草素和芒柄花素。SPE-40 含有对 HL-60 细胞增殖活性负责的主要化合物。因此，通过生物测定指导的分段过程，从 ASMq 中获得了对 HL-60 细胞具有高增殖活性的酚类部分。这可以为癌症患者提供更好的药物制剂，最大限度地减少每天服用 1.5 升（1.5 升/天）ASMq 汤剂的不便。
