Warren J B, Loi R, Rendell N B, Taylor G W
Department of Clinical Pharmacology, Royal Postgraduate Medical School, London, U.K.
Biochem J. 1990 Mar 15;266(3):921-3.
Pyocyanin is a phenazine pigment produced by the bacterium Pseudomonas aeruginosa and found in human lung secretions. Micromolar concentrations of pyocyanin inhibited the bioactivity of endothelium-derived relaxing factor (EDRF) generated from bovine pulmonary-artery endothelium in response to bradykinin. This inhibition was reversed by perfusing the EDRF-bioassay system with pyocyanin-free buffer for 15 min, but persisted in the presence of superoxide dismutase (20 units/ml). When nitric oxide, the major component of EDRF, was passed into an aqueous solution of pyocyanin in the absence of O2, a rapid colour change occurred from blue to pink; m.s. analysis of the products showed that the pyocyanin had been converted into a nitrosylated species.
绿脓杆菌素是由铜绿假单胞菌产生的一种吩嗪色素，存在于人类肺分泌物中。微摩尔浓度的绿脓杆菌素可抑制牛肺动脉内皮细胞产生的内皮源性舒张因子（EDRF）对缓激肽的生物活性。通过用不含绿脓杆菌素的缓冲液灌注EDRF生物测定系统15分钟，这种抑制作用可被逆转，但在超氧化物歧化酶（20单位/毫升）存在的情况下仍然持续。当EDRF的主要成分一氧化氮在没有O2的情况下通入绿脓杆菌素的水溶液时，会迅速发生从蓝色到粉红色的颜色变化；产物的质谱分析表明，绿脓杆菌素已转化为一种亚硝基化产物。
