Wu Jiayuan, Zhou Yuantao, Liu Yumiao, Zheng Chunfu, Li Li
Kunming Key Laboratory of Children Infection and Immunity, Yunnan Key Laboratory of Children's Major Disease Research, Yunnan Institute of Pediatrics, Yunnan Province Clinical Research Center for Children's Health and Disease, Kunming Children's Hospital, Kunming, Yunnan, China.
Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, AB, Canada.
Methods Mol Biol. 2025;2940:283-306. doi: 10.1007/978-1-0716-4615-1_25.
The viral infection of host cells is a dynamic and complex process characterized by multifaceted interactions and metabolic shifts between the virus and host. In order to better understand the molecular mechanisms involved in the viral life cycle-particularly replication, assembly, release, and the host cell response-researchers have turned to isotope labeling techniques. The employment of isotope labeling techniques as a precise method to track molecular changes is a common practice in the research community, with the aim of gaining deeper insights into the molecular mechanisms involved in the viral life cycle, including replication, assembly, release, and the host cell response. This review aims to provide a comprehensive summary of the current isotope labeling techniques commonly used for the analysis of virus-infected cells. It will also outline the relevant research protocols and their advantages and limitations. When employed in conjunction with high-resolution mass spectrometry, isotope labeling provides enhanced quantitative data, allowing researchers to track molecular dynamics in viruses and host cells with a high degree of precision.
病毒对宿主细胞的感染是一个动态且复杂的过程，其特征是病毒与宿主之间存在多方面的相互作用和代谢变化。为了更好地理解病毒生命周期中涉及的分子机制，特别是复制、组装、释放以及宿主细胞反应，研究人员已转向同位素标记技术。在研究领域，将同位素标记技术用作追踪分子变化的精确方法是一种常见做法，目的是更深入地了解病毒生命周期中涉及的分子机制，包括复制、组装、释放以及宿主细胞反应。本综述旨在全面总结目前常用于分析病毒感染细胞的同位素标记技术。它还将概述相关的研究方案及其优缺点。当与高分辨率质谱联用使用时，同位素标记可提供增强的定量数据，使研究人员能够高精度地追踪病毒和宿主细胞中的分子动态。
