Du Xusheng, Zhou Defang, Zhou Jing, Xue Jingwen, Cheng Ziqiang
College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China.
Front Vet Sci. 2022 Mar 22;9:854007. doi: 10.3389/fvets.2022.854007. eCollection 2022.
Coinfection with Marek's disease virus (MDV) and reticuloendotheliosis virus (REV) causes synergistic pathogenic effects and serious losses to the poultry industry. However, whether there is a synergism between the two viruses in viral replication and the roles of host factors in regulating MDV and REV coinfection remains elusive. In this study, we found that MDV and REV coinfection increased viral replication in coinfected cells as compared to a single infection in a limited period. Further, we explore the host cell responses to MDV and REV coinfection using tandem mass tag (TMT) peptide labeling coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Compared with MDV/REV-infected cells, 38 proteins increased (fold change > 1.2) and 60 decreased (fold change < 0.83) their abundance in MDV and REV coinfected cells. Differentially accumulated proteins (DAPs) were involved in important biological processes involved in the immune system process, cell adhesion and migration, cellular processes, and multicellular organismal systems. STRING analysis found that IRF7, MX1, TIMP3, and AKT1 may be associated with MDV and REV synergistic replication in chicken embryo fibroblasts (CEFs). Western blotting analysis showed that the selected DAPs were identical to the quantitative proteomics data. Taken together, we verified that MDV and REV can synergistically replicate in coinfected cells and revealed the host molecules involved in it. However, the synergistic pathogenesis of MDV and REV needs to be further studied.
马立克氏病病毒（MDV）与网状内皮组织增殖病病毒（REV）的共感染会产生协同致病作用，给家禽业造成严重损失。然而，这两种病毒在病毒复制过程中是否存在协同作用以及宿主因子在调节MDV和REV共感染中的作用仍不清楚。在本研究中，我们发现与单一感染相比，MDV和REV共感染在有限时间内增加了共感染细胞中的病毒复制。此外，我们使用串联质谱标签（TMT）肽标记结合液相色谱-串联质谱（LC-MS/MS）技术，探究了宿主细胞对MDV和REV共感染的反应。与MDV/REV感染的细胞相比，38种蛋白质在MDV和REV共感染的细胞中丰度增加（变化倍数>1.2），60种蛋白质丰度降低（变化倍数<0.83）。差异积累蛋白（DAPs）参与了免疫系统过程、细胞黏附与迁移、细胞过程以及多细胞生物系统等重要生物学过程。STRING分析发现，IRF7、MX1、TIMP3和AKT1可能与鸡胚成纤维细胞（CEFs）中MDV和REV的协同复制有关。蛋白质印迹分析表明，所选的DAPs与定量蛋白质组学数据一致。综上所述，我们证实了MDV和REV可在共感染细胞中协同复制，并揭示了其中涉及的宿主分子。然而，MDV和REV的协同致病机制仍需进一步研究。
