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基于 PCR 的检测方法可用于检测蜜蜂的气管螨 Acarapis woodi。

PCR-based detection of a tracheal mite of the honey bee Acarapis woodi.

机构信息

Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa, Nagoya 464-8601, Japan.

出版信息

J Invertebr Pathol. 2011 Oct;108(2):135-7. doi: 10.1016/j.jip.2011.07.009. Epub 2011 Aug 4.

DOI:10.1016/j.jip.2011.07.009
PMID:21839741
Abstract

The effects of the tracheal mite Acarapis woodi on the health of honey bees have been neglected since the prevalence of Varroa mites to Apis mellifera colonies. However, tracheal mite infestation of honey bee colonies still occurs worldwide and could impose negative impact on apiculture. The detection of A. woodi requires the dissection of honey bees followed by microscopic observation of the tracheal sacs. We thus developed PCR methods to detect A. woodi. These methods facilitate rapid and sensitive detection of A. woodi in many honey bee samples for epidemiologic surveys.

摘要

自从瓦螨(Varroa mites)普遍存在于蜜蜂(Apis mellifera)群体以来,对蜜蜂气管螨(Acarapis woodi)对蜜蜂健康的影响一直被忽视。然而,气管螨对蜜蜂群体的侵袭仍在世界范围内发生,并可能对养蜂业造成负面影响。检测 A. woodi 需要对蜜蜂进行解剖,然后对气管囊中进行显微镜观察。因此,我们开发了用于检测 A. woodi 的 PCR 方法。这些方法有助于在许多蜜蜂样本中快速、灵敏地检测 A. woodi,以进行流行病学调查。

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