Bee Pathology Laboratory, Centro Apícola Regional, Junta de Comunidades de Castilla La Mancha, 19180 Marchamalo, Spain.
Exp Parasitol. 2012 Dec;132(4):530-6. doi: 10.1016/j.exppara.2012.08.018. Epub 2012 Sep 10.
Acarapis woodi is an internal obligate parasite of the respiratory system of honey bees which provokes significant economic losses in many geographical areas. The main aim of this study was assess the A. woodi role in the "higher honey bee colony losses phenomenon" in Spain. Therefore, a new polymerase chain reaction (PCR) was developed to amplify the mitochondrial cytochrome oxidase I gene (COI) and so the actual prevalence of A. woodi in Spanish honey bee colonies in 2006 and 2007 was determined as part of a wider survey. The results revealed a greater prevalence than expected in most of the geographical areas studied where has been generally underestimated One problem encountered in this study was to distinguish between A. woodi and other species (Acarapis dorsalis and Acarapis externus) at the molecular level. Furthermore, the patterns of genetic divergence across sequences raised serious doubts about the current classification of these organisms.
Acarapis woodi 是一种蜜蜂呼吸系统的内寄生性专性寄生虫，在许多地理区域都造成了巨大的经济损失。本研究的主要目的是评估 A. woodi 在西班牙“更高的蜜蜂群损失现象”中的作用。因此，开发了一种新的聚合酶链反应（PCR）来扩增线粒体细胞色素氧化酶 I 基因（COI），从而确定了 2006 年和 2007 年西班牙蜜蜂群中 A. woodi 的实际流行率，作为更广泛调查的一部分。结果显示，在大多数研究的地理区域，其流行率高于预期，而此前这些区域的流行率一直被低估。本研究中遇到的一个问题是在分子水平上区分 A. woodi 和其他物种（Acarapis dorsalis 和 Acarapis externus）。此外，跨序列的遗传分歧模式严重怀疑这些生物体的当前分类。
