Technical University of Denmark, Department of Micro- and Nanotechnology, Oersteds Plads 345 East, DK-2800 Kongens Lyngby, Denmark.
Biosens Bioelectron. 2011 Oct 15;28(1):386-92. doi: 10.1016/j.bios.2011.07.053. Epub 2011 Jul 28.
The demand in the field of medical diagnostics for simple, cost efficient and disposable devices is growing. Here, we present a label free, all-polymer electrochemical biosensor for detection of acute viral disease. The dynamics of a viral infection in human cell culture was investigated in a micro fluidic system on conductive polymer PEDOT:TsO microelectrodes by electrochemical impedance spectroscopy and video time lapse microscopy. Employing this sensitive, real time electrochemical technique, we could measure the immediate cell response to cytomegalovirus, and detect an infection within 3h, which is several hours before the cytopathic effect is apparent with conventional imaging techniques. Atomic force microscopy and scanning ion conductance microscopy imaging consolidate the electrochemical measurements by demonstrating early virus induced changes in cell morphology of apparent programmed cell death.
医疗诊断领域对简单、经济高效且一次性使用设备的需求正在增长。在这里，我们展示了一种无标记、全聚合物电化学生物传感器，用于检测急性病毒疾病。通过电化学阻抗谱和视频延时显微镜，在微流控系统中研究了人类细胞培养中病毒感染的动力学。通过使用这种灵敏的实时电化学技术，我们可以测量巨细胞病毒对细胞的即时反应，并在细胞病变效应明显之前，通过传统的成像技术在 3 小时内检测到感染。原子力显微镜和扫描离子电导显微镜成像通过显示早期病毒诱导的细胞形态明显程序性细胞死亡的变化，巩固了电化学测量。
