Technical University of Denmark, Department of Micro- and Nanotechnology, Oersteds Plads 345 East, DK-2800 Kongens Lyngby, Denmark.
Biosens Bioelectron. 2013 Nov 15;49:374-9. doi: 10.1016/j.bios.2013.05.046. Epub 2013 Jun 10.
Influenza infections are associated with high morbidity and mortality, carry the risk of pandemics, and pose a considerable economic burden worldwide. To improve the management of the illness, it is essential with accurate and fast point-of-care diagnostic tools for use in the field or at the patient's bedside. Conventional diagnostic methods are time consuming, expensive and require specialized laboratory facilities. We present a highly sensitive, highly specific, and low cost platform to test for acute virus infections in less than 15 min, employing influenza A virus (H1N1) as an example of its usability. An all polymer microfluidic system with a functionalized conductive polymer (PEDOT-OH:TsO) microelectrode array was developed and exploited for label free and real time electrochemical detection of intact influenza A virus (H1N1) particles. DNA aptamers with affinity for influenza A virus (H1N1) were linked covalently to the conductive polymer microelectrodes in the microfluidic channel. Based on changes in the impedance when virions were captured by immobilized probes, we could detect clinically relevant concentrations of influenza A virus (H1N1) in saliva. This is a new, stable and very sensitive point-of-care platform for detection and diagnostics of intact virus particles.
流感感染与高发病率和死亡率相关，有引发大流行的风险，并在全球范围内造成相当大的经济负担。为了改善疾病管理，使用现场或患者床边的准确、快速的即时诊断工具至关重要。传统的诊断方法既耗时又昂贵，且需要专门的实验室设备。我们提出了一种高灵敏度、高特异性且低成本的平台，用于在 15 分钟内检测急性病毒感染，以流感 A 病毒 (H1N1) 为例证明其可用性。我们开发并利用带有功能化导电聚合物 (PEDOT-OH:TsO) 微电极阵列的全聚合物微流控系统，用于无标记和实时电化学生物传感器检测完整的流感 A 病毒 (H1N1) 颗粒。具有流感 A 病毒 (H1N1) 亲和力的 DNA 适体通过共价键连接到微流控通道中的导电聚合物微电极上。基于病毒粒子被固定探针捕获时阻抗的变化，我们可以检测到唾液中临床相关浓度的流感 A 病毒 (H1N1)。这是一种用于检测和诊断完整病毒颗粒的新型、稳定且非常灵敏的即时诊断平台。
