School of Veterinary Sciences, Universidad Nacional de Rosario, Casilda, Argentina.
Immunotherapy. 2011 Aug;3(8):971-8. doi: 10.2217/imt.11.93.
Can heat-killed, borate-buffered suspensions of Gordonia bronchialis, Rhodococcus coprophilus or Tsukamurella inchonensis be used to treat canine flea allergy?
MATERIALS & METHODS: Organisms cultured on Sauton's medium into stationary phase were autoclaved in borate-buffered saline and stored at 10 mg wet weight/ml. Intradermal injections of 0.1 ml containing 1 mg of bacilli were administered on the first and 20th days of the study. G. bronchialis and R. coprophilus were most effective in a pilot study of a small number of dogs with flea allergy. A larger number of affected dogs were then randomized to receive placebo or either of the two selected reagents. The extent and severity of allergic signs and symptoms were scored and blood samples were collected just before the first injection and 28 days after the second.
Both selected reagents reduced the extent and severity of lesions (p < 0.001) and reduced scratching. Eosinophil numbers were reduced (p < 0.0001) between the first and second assessment.
Injections of G. bronchialis or R. coprophilus effectively reduce the signs and symptoms of flea allergy in dogs.
经热处理并用硼酸缓冲液制成的戈登氏分枝杆菌、解糖假丝酵母或印度生驹氏菌混悬液能否用于治疗犬跳蚤过敏症？
将在 Sauton 培养基上培养至静止期的生物体在硼酸缓冲盐溶液中进行高压灭菌，以 10mg 湿重/ml 的浓度保存。在研究的第 1 天和第 20 天，通过皮内注射 0.1ml 含有 1mg 杆菌的溶液。在一项对少数患有跳蚤过敏症的犬进行的初步研究中，G. bronchialis 和 R. coprophilus 最为有效。然后，更多受影响的犬被随机分为接受安慰剂或两种选定试剂中的一种。对过敏症状的严重程度和严重程度进行评分，并在第一次注射前和第二次注射后 28 天采集血样。
两种选定的试剂均能减轻病变的严重程度（p<0.001）和减少瘙痒。在第一次和第二次评估之间，嗜酸性粒细胞数量减少（p<0.0001）。
注射戈登氏分枝杆菌或解糖假丝酵母可有效减轻犬跳蚤过敏的症状。
