Nofouzi Katayoon, Sheikhzadeh Najmeh, Varshoie Hossein, Sharabyani Sona Khadir, Jafarnezhad Mehran, Shabanzadeh Sadigheh, Ahmadifar Ehsan, Stanford John, Shahbazfar Amir Ali
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Department of Food Hygiene and Aquatic Animals, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Fish Physiol Biochem. 2019 Feb;45(1):209-217. doi: 10.1007/s10695-018-0555-4. Epub 2018 Sep 3.
Present study was conducted to investigate the effects of heat-killed Tsukamurella inchonensis on growth performance, gastrointestinal structure, immune response, and biochemical parameters in rainbow trout. Fish (mean weight 25 g) were fed basal diet (control), diets containing 2.48 × 10 colony-forming units (low-dose group) or 1.24 × 10 colony-forming units (high-dose group) of heat-killed Tsukamurella inchonensis per 1 kg of feed for 90 days. Results showed that growth performance was significantly enhanced in both treatment groups compared to the control group. The intestinal villus length and pyloric cecal fold length were mainly enhanced in the high-dose group. On the other hand, higher goblet cell percentage was shown with administration of dead Tsukamurella inchonensis in both treatment groups. Immune parameters such as alternative complement activity, immunoglobulin level, and hemagglutination titer were significantly higher in treatment groups than in fish fed in the control diet. Meanwhile, feeding heat-killed Tsukamurella inchonensis especially at higher dose caused a decrease in the levels of total cholesterol, uric acid, and lipid peroxidation product whereas no significant changes were noted in serum-specific marker enzymes levels, namely alanine aminotransferase (ALT), alkaline phosphatase (ALP), and aspartate aminotransferase (AST) by feeding both treatment diets compared to the control group. This study suggests that heat-killed Tsukamurella inchonensis especially at 1.24 × 10 colony-forming units had more potential to enhance growth, immunological parameters, and intestinal structure in rainbow trout.
本研究旨在探讨热灭活的仁川冢村菌对虹鳟生长性能、胃肠道结构、免疫反应及生化参数的影响。将平均体重25克的鱼分为三组，分别投喂基础饲料（对照组）、每千克饲料含2.48×10菌落形成单位的热灭活仁川冢村菌的饲料（低剂量组）或每千克饲料含1.24×10菌落形成单位的热灭活仁川冢村菌的饲料（高剂量组），持续投喂90天。结果显示，与对照组相比，两个处理组的生长性能均显著提高。高剂量组的肠道绒毛长度和幽门盲囊褶皱长度主要增加。另一方面，两个处理组在投喂灭活的仁川冢村菌后杯状细胞百分比均较高。处理组的替代补体活性、免疫球蛋白水平和血凝效价等免疫参数显著高于投喂对照饲料的鱼。同时，投喂热灭活的仁川冢村菌，尤其是高剂量时，会导致总胆固醇、尿酸和脂质过氧化产物水平降低，而与对照组相比，投喂两种处理饲料后血清特异性标志物酶水平，即丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）和天冬氨酸转氨酶（AST）均无显著变化。本研究表明，热灭活的仁川冢村菌，尤其是1.24×10菌落形成单位时，对提高虹鳟的生长、免疫参数和肠道结构具有更大的潜力。
