Cátedra de Biología, Facultad de Ciencias Medicas, Universidad Nacional de Rosario, CIUNR, Santa Fe, Rosario, Argentina.
Immunotherapy. 2012 May;4(5):487-97. doi: 10.2217/imt.12.37.
To determine whether immunotherapy with heat-killed, selected Actinomycetales species could influence the progression of spontaneous Type 2 diabetes mellitus and obesity in a rat model.
MATERIALS & METHODS: Preparations of either Gordonia bronchialis, Tsukamurella inchonensis or a saline placebo were given by three subcutaneous injections, 30 days apart, starting when rats were aged 120 days, just before development of Type 2 diabetes mellitus, and at day 440, when the disease was well established. Bodyweight, blood sugar, cholesterol, triglycerides and insulin levels were measured to determine the effects and at the end of the experiments, animals were subjected to necropsy.
The development of Type 2 diabetes mellitus was prevented by both reagents, most effectively by T. inchonensis. In the treatment experiment, the effects of the disease were reduced by both treatments, markedly so by T. inchonensis. In both experiments obesity was reduced in treated animals. The possible mechanisms of action are discussed.
Our findings suggest that Type 2 diabetes mellitus in the studied rats is associated with obesity, and that both diabetes and obesity can be prevented or improved by treatment with Actinomycetales immune modulators.
确定经热灭活、选择性放线菌类物种的免疫疗法是否会影响自发性 2 型糖尿病和肥胖在大鼠模型中的进展。
当大鼠 120 天大，即 2 型糖尿病发病前，以及疾病已确立的第 440 天，通过 3 次皮下注射，每隔 30 天给予 Gordonia bronchialis、Tsukamurella inchonensis 或盐水安慰剂制剂。测量体重、血糖、胆固醇、甘油三酯和胰岛素水平，以确定作用，在实验结束时，对动物进行尸检。
两种试剂均预防了 2 型糖尿病的发生，Tsukamurella inchonensis 的效果最为显著。在治疗实验中，两种治疗均减轻了疾病的影响，Tsukamurella inchonensis 的效果更为明显。在这两种实验中，治疗组的肥胖均得到减轻。讨论了可能的作用机制。
我们的研究结果表明，所研究大鼠的 2 型糖尿病与肥胖有关，通过使用放线菌类免疫调节剂进行治疗，可以预防或改善糖尿病和肥胖。
