Department of Animal Biotechnology, University of Technology and Life Sciences, Mazowiecka 28, 85-225 Bydgoszcz, Poland.
Poult Sci. 2011 Sep;90(9):1909-15. doi: 10.3382/ps.2011-01465.
Natural antibodies (NA) create a crucial barrier at the initial steps of the innate humoral immune response. The main role of NA in the defense system is to bind the pathogens at early stages of infection. Different pathogens are recognized by the presence of highly conserved antigen determinant [e.g., lipopolysaccharide (LPS) in gram-negative bacteria or lipoteichoic acid (LTA) in gram-positive bacteria]. In chickens, a different genetic background of NA binds LPS and LTA antigens, encoded by different QTL. The main objective of this work was to confirm known QTL associated with LPS and LTA NA. For this purpose a chicken reference population was created by crossing 2 breeds: a commercial layer, White Leghorn, and a Polish indigenous chicken, Green-Legged Partridgelike. The chromosomal regions analyzed harbored to GGA3, GGA5, GGA6, GGA8, GGA9, GGA10, GGA14, GGA15, GGA18, and GGAZ. The data collected consisted of the NA titers binding LPS and LTA (determined by ELISA at 12 wk of age) as well as the genotypes (30 short tandem repeat markers; average of 3 markers/chromosome, collected for generations F(0), F(1), and F(2)). The analyses were performed with 3 statistical models (paternal and maternal half-sib, line cross, and linkage analysis and linkage disequilibrium) implemented in GridQTL software (http://www.gridqtl.org.uk/). The QTL study of humoral innate immune response traits resulted in the confirmation of 3 QTL associated with NA titers binding LPS (located on GGA9, GGA18, and GGAZ) and 2 QTL associated with NA titers binding LTA (located on GGA5 and GGA14). A set of candidate genes within the regions of the validated QTL has been proposed.
天然抗体 (NA) 在先天体液免疫反应的初始步骤中形成了一个关键的屏障。NA 在防御系统中的主要作用是在感染的早期阶段结合病原体。不同的病原体通过存在高度保守的抗原决定簇来识别，例如革兰氏阴性菌中的脂多糖 (LPS) 或革兰氏阳性菌中的脂磷壁酸 (LTA)。在鸡中，不同遗传背景的 NA 结合 LPS 和 LTA 抗原，这些抗原由不同的 QTL 编码。这项工作的主要目的是确认与 LPS 和 LTA NA 相关的已知 QTL。为此，通过杂交 2 个品种：商业蛋鸡，白来航鸡，和波兰本土鸡，绿腿鹧鸪样鸡，创建了一个鸡参考群体。分析的染色体区域包含 GGA3、GGA5、GGA6、GGA8、GGA9、GGA10、GGA14、GGA15、GGA18 和 GGAZ。收集的数据包括结合 LPS 和 LTA 的 NA 滴度（通过 ELISA 在 12 周龄时测定）以及基因型（30 个短串联重复标记；每个染色体平均 3 个标记，在 F(0)、F(1) 和 F(2) 代收集）。使用 3 种统计模型（父系和母系半同胞、系杂交和连锁分析以及连锁不平衡）在 GridQTL 软件（http://www.gridqtl.org.uk/）中进行了分析。对体液先天免疫反应性状的 QTL 研究证实了与结合 LPS 的 NA 滴度相关的 3 个 QTL（位于 GGA9、GGA18 和 GGAZ）和与结合 LTA 的 NA 滴度相关的 2 个 QTL（位于 GGA5 和 GGA14）。已经提出了在验证的 QTL 区域内的一组候选基因。
