Key Laboratory of Bio-Resources and Eco-Environment (Ministry of Education), Sichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Science, Sichuan University, 610064 Chengdu, PR China.
Key Laboratory of Bio-Resources and Eco-Environment (Ministry of Education), Bioengineering Research Center for Animal Disease Prevention and Control, College of Life Science, Sichuan University, 610064 Chengdu, PR China.
J Med Microbiol. 2011 Dec;60(Pt 12):1820-1826. doi: 10.1099/jmm.0.033332-0. Epub 2011 Aug 18.
Arcanobacterium pyogenes is commonly isolated from ruminant animals as an opportunistic pathogen that co-infects with other bacteria, normally causing surface or internal abscesses. Twenty-eight strains of A. pyogenes isolated from forest musk deer suppurative samples were identified by their 16S rRNA gene sequences, and confirmed by amplification of the pyolysin-encoding gene (plo) in all isolates. The MICs of 14 commonly used antibiotics were determined by an agar dilution method. Class 1 and 2 intI genes were amplified to determine whether integrons were present in the A. pyogenes genome. Class 1 gene cassettes were detected by specific primers and analysed by sequencing. All of the strains were susceptible to most fluoroquinolone antibiotics; however, high resistance rates were observed for β-lactams and trimethoprim. A total of 18 of the isolates (64.3%) were positive for the class 1 intI gene, and 16 (57.1%) contained class 1 gene cassettes with the aacC, aadA1, aadA2, blaP1 and dfr2a genes. Most were present in the multi-resistant isolates, indicating a general concordance between the presence of gene cassettes and antibiotic resistance, and that the integrons have played an important role in the dissemination of antimicrobial resistance in this species.
化脓隐秘杆菌通常作为一种机会性病原体从反刍动物中分离出来，与其他细菌共同感染，通常导致表面或内部脓肿。从林麝化脓性样本中分离的 28 株化脓隐秘杆菌通过 16S rRNA 基因序列进行鉴定，并通过所有分离株中编码 pyolysin 的基因（plo）的扩增得到确认。采用琼脂稀释法测定了 14 种常用抗生素的 MIC。扩增 1 类和 2 类 intI 基因以确定整合子是否存在于化脓隐秘杆菌基因组中。通过特异性引物扩增检测到 1 类基因盒，并进行测序分析。所有菌株对大多数氟喹诺酮类抗生素敏感；然而，β-内酰胺类和甲氧苄啶的耐药率较高。18 株（64.3%）分离株对 1 类 intI 基因呈阳性，16 株（57.1%）含有 aacC、aadA1、aadA2、blaP1 和 dfr2a 基因的 1 类基因盒。大多数基因盒存在于多耐药株中，这表明基因盒的存在与抗生素耐药性之间存在一般一致性，并且整合子在该物种中抗微生物耐药性的传播中发挥了重要作用。
