用于检测大肠杆菌热稳定肠毒素的竞争性酶免疫分析试剂盒与幼鼠试验的比较。
Comparison of a competitive enzyme immunoassay kit and the infant mouse assay for detecting Escherichia coli heat-stable enterotoxin.
作者信息
Stavric S, Buchanan B, Speirs J
机构信息
Bureau of Microbial Hazards, Health & Welfare Canada, Ottawa, Ontario.
出版信息
Lett Appl Microbiol. 1992 Feb;14(2):47-50. doi: 10.1111/j.1472-765x.1992.tb00644.x.
Abstract
A commercial competitive enzyme immunoassay kit, Escherichia coli ST EIA, was compared with the conventional infant mouse assay for sensitivity and specificity in detecting E. coli heat-stable enterotoxin. Thirty-one of 46 strains of E. coli tested were positive by both assays, while 15 strains were negative. The sensitivity of the ST EIA kit was up to 64-fold lower than the infant mouse assay.
摘要
一种商业竞争性酶免疫分析试剂盒——大肠杆菌ST酶免疫分析试剂盒，与传统的幼鼠检测法在检测大肠杆菌热稳定肠毒素方面的敏感性和特异性进行了比较。在检测的46株大肠杆菌中，两种检测方法均为阳性的有31株，15株为阴性。ST酶免疫分析试剂盒的敏感性比幼鼠检测法低达64倍。
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