通过一种市售酶联免疫测定法鉴定产热稳定肠毒素STA的大肠杆菌，并将该测定法与幼鼠试验和DNA探针试验进行比较。
Identification of Escherichia coli that produces heat-stable enterotoxin STA by a commercially available enzyme-linked immunoassay and comparison of the assay with infant mouse and DNA probe tests.
作者信息
Scotland S M, Willshaw G A, Said B, Smith H R, Rowe B
机构信息
Division of Enteric Pathogens, Central Public Health Laboratory, London, England.
出版信息
J Clin Microbiol. 1989 Jul;27(7):1697-9. doi: 10.1128/jcm.27.7.1697-1699.1989.
Abstract
By using the infant mouse test and a commercially available competitive enzyme-linked immunosorbent assay (ELISA), 100 strains of Escherichia coli carrying STA1 or STA2 genes were shown to produce the heat-stable enterotoxin STA. An additional 100 strains were negative in both tests. The ELISA was easy to perform, and results were available within 24 h. Testing strains with an enzyme-linked DNA probe kit that incorporated both STA1- and STA2-specific oligonucleotides showed that the 100 strains positive in the mouse test and ELISA also hybridized with the mixed probe. Two strains carrying STA1 genes but negative in the mouse test and ELISA also hybridized with the mixed probe.
摘要
通过使用幼鼠试验和市售的竞争性酶联免疫吸附测定（ELISA），结果显示携带STA1或STA2基因的100株大肠杆菌可产生热稳定肠毒素STA。另外100株在两种试验中均呈阴性。ELISA操作简便，24小时内即可获得结果。用包含STA1和STA2特异性寡核苷酸的酶联DNA探针试剂盒检测菌株，结果表明在小鼠试验和ELISA中呈阳性的100株菌株也能与混合探针杂交。两株携带STA1基因但在小鼠试验和ELISA中呈阴性的菌株也能与混合探针杂交。
相似文献
1
Identification of Escherichia coli that produces heat-stable enterotoxin STA by a commercially available enzyme-linked immunoassay and comparison of the assay with infant mouse and DNA probe tests.通过一种市售酶联免疫测定法鉴定产热稳定肠毒素STA的大肠杆菌，并将该测定法与幼鼠试验和DNA探针试验进行比较。
J Clin Microbiol. 1989 Jul;27(7):1697-9. doi: 10.1128/jcm.27.7.1697-1699.1989.
2
Blinded, two-laboratory comparative analysis of Escherichia coli heat-stable enterotoxin production by using monoclonal antibody enzyme-linked immunosorbent assay, radioimmunoassay, suckling mouse assay, and gene probes.采用单克隆抗体酶联免疫吸附测定法、放射免疫测定法、乳鼠试验和基因探针，对大肠杆菌热稳定肠毒素产生情况进行双实验室盲法比较分析。
J Clin Microbiol. 1986 Nov;24(5):753-8. doi: 10.1128/jcm.24.5.753-758.1986.
3
Comparison of three assay systems for detection of enterotoxigenic Escherichia coli heat-stable enterotoxin.三种检测产肠毒素大肠杆菌热稳定肠毒素检测系统的比较。
J Clin Microbiol. 1990 Apr;28(4):792-4. doi: 10.1128/jcm.28.4.792-794.1990.
4
Comparison of a competitive enzyme immunoassay kit and the infant mouse assay for detecting Escherichia coli heat-stable enterotoxin.用于检测大肠杆菌热稳定肠毒素的竞争性酶免疫分析试剂盒与幼鼠试验的比较。
Lett Appl Microbiol. 1992 Feb;14(2):47-50. doi: 10.1111/j.1472-765x.1992.tb00644.x.
5
Detection of heat-stable enterotoxigenic Escherichia coli by hybridization with an RNA transcript probe.用RNA转录探针杂交法检测耐热肠毒素性大肠杆菌
J Clin Microbiol. 1987 Aug;25(8):1572-3. doi: 10.1128/jcm.25.8.1572-1573.1987.
6
Type II heat-labile enterotoxin-producing Escherichia coli isolated from animals and humans.从动物和人类中分离出的产Ⅱ型热不稳定肠毒素大肠杆菌。
Infect Immun. 1988 May;56(5):1158-61. doi: 10.1128/iai.56.5.1158-1161.1988.
7
Detection of Escherichia coli and identification of enterotoxigenic strains by primer-directed enzymatic amplification of specific DNA sequences.通过引物定向酶促扩增特定DNA序列检测大肠杆菌并鉴定产肠毒素菌株。
Int J Food Microbiol. 1991 Apr;12(4):339-51. doi: 10.1016/0168-1605(91)90148-i.
8
A competitive immunosorbent assay for the detection of heat-stable enterotoxin of Escherichia coli.一种用于检测大肠杆菌热稳定肠毒素的竞争性免疫吸附测定法。
J Med Microbiol. 1985 Aug;20(1):69-74. doi: 10.1099/00222615-20-1-69.
9
Escherichia coli heat-stable enterotoxin (STa)-biotin enzyme-linked immunosorbent assay (STa-biotin ELISA).大肠杆菌热稳定肠毒素（STa）-生物素酶联免疫吸附测定（STa-生物素酶联免疫吸附测定法）
J Immunol Methods. 1994 Jul 12;173(1):1-5. doi: 10.1016/0022-1759(94)90275-5.
10
Heat-stable-enterotoxin-producing Escherichia coli strains isolated from dogs.
J Clin Microbiol. 1988 Dec;26(12):2564-6. doi: 10.1128/jcm.26.12.2564-2566.1988.
引用本文的文献
1
Nissui glucose fermentative gram-negative rod identification system EB-20 gives a unique profile for typical non-sorbitol-fermenting Escherichia coli O157:H7.日水葡萄糖发酵革兰氏阴性杆菌鉴定系统EB - 20为典型的非山梨醇发酵大肠杆菌O157:H7提供了独特的谱图。
J Clin Microbiol. 2004 Jan;42(1):354-8. doi: 10.1128/JCM.42.1.354-358.2004.
2
Full capacity of recombinant Escherichia coli heat-stable enterotoxin fusion proteins for extracellular secretion, antigenicity, disulfide bond formation, and activity.重组大肠杆菌热稳定肠毒素融合蛋白的胞外分泌、抗原性、二硫键形成及活性的全部能力。
Infect Immun. 2000 Jul;68(7):4064-74. doi: 10.1128/IAI.68.7.4064-4074.2000.
3
Evaluation of non-radioactive trivalent DNA probe (LT, ST1a, ST1b) for detecting enterotoxigenic Escherichia coli.用于检测产肠毒素大肠杆菌的非放射性三价DNA探针（LT、ST1a、ST1b）的评估
J Clin Pathol. 1993 Apr;46(4):309-12. doi: 10.1136/jcp.46.4.309.
4
Epitope mapping and characterization of antigenic determinants of heat-stable enterotoxin (STh) of enterotoxigenic Escherichia coli by using monoclonal antibodies.利用单克隆抗体对产肠毒素大肠杆菌热稳定肠毒素（STh）的抗原决定簇进行表位作图和特性分析。
Infect Immun. 1993 Jan;61(1):289-94. doi: 10.1128/iai.61.1.289-294.1993.
5
Differentiation of pathogenic Escherichia coli strains in Brazilian children by PCR.通过聚合酶链反应对巴西儿童致病性大肠杆菌菌株进行鉴别
J Clin Microbiol. 1995 May;33(5):1371-4. doi: 10.1128/jcm.33.5.1371-1374.1995.
6
Some infectious causes of diarrhea in young farm animals.幼龄农场动物腹泻的一些传染性病因。
Clin Microbiol Rev. 1990 Oct;3(4):345-75. doi: 10.1128/CMR.3.4.345.
7
Comparison of preservation methods for enterotoxigenic Escherichia coli producing heat-labile enterotoxin.产不耐热肠毒素的产肠毒素大肠杆菌保存方法的比较
J Clin Microbiol. 1991 Oct;29(10):2326-8. doi: 10.1128/jcm.29.10.2326-2328.1991.
本文引用的文献
1
Identification of enterotoxigenic Escherichia coli by colony hybridization using three enterotoxin gene probes.使用三种肠毒素基因探针通过菌落杂交鉴定产肠毒素大肠杆菌
J Infect Dis. 1982 Jun;145(6):863-9. doi: 10.1093/infdis/145.6.863.
2
Development of a radioimmunoassay for Escherichia coli heat-stable enterotoxin: comparison with the suckling mouse bioassay.一种用于检测大肠杆菌热稳定肠毒素的放射免疫测定法的开发：与乳鼠生物测定法的比较。
Infect Immun. 1981 Jul;33(1):186-92. doi: 10.1128/iai.33.1.186-192.1981.
3
Enterotoxigenic Escherichia coli (ETEC) isolated in the Tel-Aviv (Israel) area.
Med Microbiol Immunol. 1980;169(1):53-61. doi: 10.1007/BF02123712.
4
Induction of immunity against E. coli ST-enterotoxin.诱导针对大肠杆菌ST-肠毒素的免疫力。
Zentralbl Veterinarmed B. 1982 Jul;29(6):441-50. doi: 10.1111/j.1439-0450.1982.tb01246.x.
5
Acquisition and maintenance of enterotoxin plasmids in wild-type strains of Escherichia coli.大肠杆菌野生型菌株中肠毒素质粒的获得与维持
J Gen Microbiol. 1983 Oct;129(10):3111-20. doi: 10.1099/00221287-129-10-3111.
6
Prospective study of diarrheal illnesses in northeastern Brazil: patterns of disease, nutritional impact, etiologies, and risk factors.巴西东北部腹泻疾病的前瞻性研究：疾病模式、营养影响、病因及风险因素。
J Infect Dis. 1983 Dec;148(6):986-97. doi: 10.1093/infdis/148.6.986.
7
Identification of enterotoxigenic Escherichia coli in patients with diarrhea in Asia with three enterotoxin gene probes.用三种肠毒素基因探针鉴定亚洲腹泻患者中的产肠毒素大肠杆菌。
Infect Immun. 1983 Oct;42(1):152-5. doi: 10.1128/iai.42.1.152-155.1983.
8
Isolation and nucleotide sequence determination of a gene encoding a heat-stable enterotoxin of Escherichia coli.大肠杆菌热稳定肠毒素编码基因的分离与核苷酸序列测定
Infect Immun. 1983 Mar;39(3):1167-74. doi: 10.1128/iai.39.3.1167-1174.1983.
9
Immunological properties of Escherichia coli heat-stable enterotoxins: development of a radioimmunoassay specific for heat-stable enterotoxins with suckling mouse activity.大肠杆菌热稳定肠毒素的免疫学特性：针对具有乳鼠活性的热稳定肠毒素的放射免疫测定法的开发。
Infect Immun. 1981 Jul;33(1):193-8. doi: 10.1128/iai.33.1.193-198.1981.
10
Identification of enterotoxigenic Escherichia coli and serum antitoxin activity by the vascular permeability factor assay.通过血管通透性因子测定法鉴定产肠毒素大肠杆菌及血清抗毒素活性
Infect Immun. 1973 Nov;8(5):731-5. doi: 10.1128/iai.8.5.731-735.1973.
Zotero 插件