Abteilung für Molekulare Genetik, Institut für Mikrobiologie und Genetik, Göttinger Zentrum für Molekulare Biowissenschaften, Georg-August Universität Göttingen, Grisebachstrasse 8, Göttingen 37077, Germany.
EMBO Rep. 2011 Sep 30;12(10):1024-31. doi: 10.1038/embor.2011.155.
The nuclear export of large ribonucleoparticles is complex and requires specific transport factors. Messenger RNAs are exported through the RNA-binding protein Npl3 and the interacting export receptor Mex67. Export of large ribosomal subunits also requires Mex67; however, in this case, Mex67 binds directly to the 5S ribosomal RNA (rRNA) and does not require the Npl3 adaptor. Here, we have discovered a new function of Npl3 in mediating the export of pre-60S ribosomal subunit independently of Mex67. Npl3 interacts with the 25S rRNA, ribosomal and ribosome-associated proteins, as well as with the nuclear pore complex. Mutations in NPL3 lead to export defects of the large subunit and genetic interactions with other pre-60S export factors.
核输出大型核糖核蛋白复合物是一个复杂的过程，需要特定的运输因子。信使 RNA 是通过 RNA 结合蛋白 Npl3 和相互作用的输出受体 Mex67 输出的。大亚基核糖体的输出也需要 Mex67；然而，在这种情况下，Mex67 直接结合 5S 核糖体 RNA（rRNA），不需要 Npl3 衔接子。在这里，我们发现了 Npl3 的一个新功能，即在不依赖 Mex67 的情况下，介导前 60S 核糖体亚基的输出。Npl3 与 25S rRNA、核糖体和核糖体相关蛋白以及核孔复合物相互作用。NPL3 中的突变导致大亚基的输出缺陷，并与其他前 60S 输出因子发生遗传相互作用。
