mRNA 输出因子 Npl3 介导大亚基核糖体的核输出。
The mRNA export factor Npl3 mediates the nuclear export of large ribosomal subunits.
机构信息
Abteilung für Molekulare Genetik, Institut für Mikrobiologie und Genetik, Göttinger Zentrum für Molekulare Biowissenschaften, Georg-August Universität Göttingen, Grisebachstrasse 8, Göttingen 37077, Germany.
出版信息
EMBO Rep. 2011 Sep 30;12(10):1024-31. doi: 10.1038/embor.2011.155.
Abstract
The nuclear export of large ribonucleoparticles is complex and requires specific transport factors. Messenger RNAs are exported through the RNA-binding protein Npl3 and the interacting export receptor Mex67. Export of large ribosomal subunits also requires Mex67; however, in this case, Mex67 binds directly to the 5S ribosomal RNA (rRNA) and does not require the Npl3 adaptor. Here, we have discovered a new function of Npl3 in mediating the export of pre-60S ribosomal subunit independently of Mex67. Npl3 interacts with the 25S rRNA, ribosomal and ribosome-associated proteins, as well as with the nuclear pore complex. Mutations in NPL3 lead to export defects of the large subunit and genetic interactions with other pre-60S export factors.
摘要
核输出大型核糖核蛋白复合物是一个复杂的过程，需要特定的运输因子。信使 RNA 是通过 RNA 结合蛋白 Npl3 和相互作用的输出受体 Mex67 输出的。大亚基核糖体的输出也需要 Mex67；然而，在这种情况下，Mex67 直接结合 5S 核糖体 RNA（rRNA），不需要 Npl3 衔接子。在这里，我们发现了 Npl3 的一个新功能，即在不依赖 Mex67 的情况下，介导前 60S 核糖体亚基的输出。Npl3 与 25S rRNA、核糖体和核糖体相关蛋白以及核孔复合物相互作用。NPL3 中的突变导致大亚基的输出缺陷，并与其他前 60S 输出因子发生遗传相互作用。
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