曼氏无针乌贼（头足纲；乌贼科）新多态微卫星标记的分离与鉴定

Isolation and characterization of new polymorphic microsatellite markers from the cuttlefish Sepiella maindroni (Cephalopoda; Sepiidae).

作者信息

Guo B-Y, Qi P Z, Zhu A Y, Lv Z M, Wang W C, Wu C W

机构信息

Zhejiang Ocean University, National Engineering Research Center of Marine Facilities Aquaculture, Zhoushan, China.

出版信息

Genet Mol Res. 2013 Jul 11;12(3):2376-9. doi: 10.4238/2013.July.11.1.

DOI:10.4238/2013.July.11.1

PMID:23884774

Abstract

Fifteen new polymorphic microsatellite loci were developed for the cuttlefish Sepiella maindroni. In 32 individuals from a wild population of coastal Ningde, Fujian Province, China, the number of alleles at these loci varied between 2 and 12, with an average of 5.86. The mean observed and expected heterozygosities were 0.6917 and 0.5993, respectively. Among these polymorphic microsatellite loci, 4 (SM2, SM19, SM40, and SM81) significantly deviated from Hardy-Weinberg equilibrium after sequential Bonferroni's correction. All of them were in linkage equilibrium. These microsatellite loci would be useful for evaluating the effect of releasing on extant S. maindroni populations as well as for investigating genetic diversity and population structure of this species.

摘要

为曼氏无针乌贼开发了15个新的多态性微卫星位点。在中国福建省宁德沿海的一个野生种群的32个个体中，这些位点的等位基因数量在2到12之间变化，平均为5.86。观察到的平均杂合度和预期杂合度分别为0.6917和0.5993。在这些多态性微卫星位点中，经过连续Bonferroni校正后，有4个（SM2、SM19、SM40和SM81）显著偏离哈迪-温伯格平衡。它们全部处于连锁平衡。这些微卫星位点将有助于评估放流对现存曼氏无针乌贼种群的影响，以及研究该物种的遗传多样性和种群结构。