Department of Dentistry, AJA University of Medical Sciences - Laser Research Center, Faculty of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Photodiagnosis Photodyn Ther. 2011 Sep;8(3):249-53. doi: 10.1016/j.pdpdt.2011.03.337. Epub 2011 Apr 3.
The use of photoactivated disinfection has had a significant medical and technological effect in bacterial inactivation, as an alternative to conventional antimicrobial methods. The main goal of this study was to investigate the effect of photoactivated disinfection on Streptococcus mutans, when Radachlorin(®) was used as a photosensitizer.
Streptococcus mutans samples of two different initial concentrations were treated with Radachlorin(®) gel (0.1%), irradiated by the light of a He-Ne laser (633nm), with energy density of 6J/cm(2), and cell viability was evaluated after culturing.
It was observed that the combination of Radachlorin(®) and laser was more effective than Radachlorin(®) or laser alone (p<0.05), in reduction of S. mutans and Radachlorin(®) was cytotoxic, in the dark, only for the lower concentration of bacteria. Lower concentration of S. mutans resulted in higher amount of killing, in the case of using Radachlorin(®) with or without laser.
The photoactivation of Radachlorin(®) using a He-Ne laser could inactivate S. mutans to a significant level. In addition Radachlorin(®) might be cytotoxic in the dark, for the lower concentration of bacteria.
光动力杀菌在细菌失活方面具有重要的医学和技术作用，可作为传统抗菌方法的替代方法。本研究的主要目的是研究当使用 Radachlorin(®)作为光敏剂时，光动力杀菌对变形链球菌的影响。
用 Radachlorin(®)凝胶（0.1%）处理两种初始浓度不同的变形链球菌样本，用氦氖激光（633nm）照射，能量密度为 6J/cm(2)，并在培养后评估细胞活力。
观察到 Radachlorin(®)和激光的组合比单独使用 Radachlorin(®)或激光更有效（p<0.05），可降低变形链球菌的数量，并且 Radachlorin(®)在黑暗中具有细胞毒性，仅对较低浓度的细菌具有细胞毒性。在使用 Radachlorin(®)或不使用激光的情况下，较低浓度的变形链球菌会导致更高的杀伤率。
使用氦氖激光对 Radachlorin(®)进行光激活可以使变形链球菌达到显著失活水平。此外，Radachlorin(®)在黑暗中对较低浓度的细菌可能具有细胞毒性。
