Vahabi S, Fekrazad R, Ayremlou S, Taheri S, Zangeneh N
Assistant Professor, Department of Periodontics, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Dent (Tehran). 2011 Spring;8(2):48-54. Epub 2011 Jun 30.
Dental caries and periodontal diseases are caused by infection of teeth and supporting tissues due to complex aggregate of bacteria known as biofilm, firstly colonized by streptococci. The main purpose of this in vitro study was to evaluate the antimicrobial effects of toluidine blue O (TBO) and Radachlorin® in combination with a diode laser on the viability of Streptococcus mutans.
Bacterial suspensions of Streptococcus mutans were exposed to either 0.1% TBO associated with (20 mW, 633 nm diode laser, continuous mode, 150 s) or 0.1% Radachlorin® and laser irradiation (100 mW, 662 nm diode laser, continuous mode, 120 s). Those in control groups were subjected to laser irradiation alone or TBO/Radachlorin® alone or received neither TBO/Radachlorin® nor laser exposure. The suspensions were then spread over specific agar plates and incubated aerobically at 37°C. Finally, the bactericidal effects were evaluated based on colony formation.
Potential bacterial cell killing was only observed following photosensitization with TBO and 3 j/cm(2) laser exposure (p<0.05), whereas Radachlorin® showed significant reduction in dark condition compared to laser exposure (p<0.05).
TBO-mediated photodynamic therapy seems to be more efficient than Radachlorin® in significantly reducing the viability of Streptococcus mutans in vitro.
龋齿和牙周疾病是由一种称为生物膜的复杂细菌聚集体感染牙齿及支持组织所致，最初定殖的是链球菌。本体外研究的主要目的是评估甲苯胺蓝O（TBO）和卟吩姆钠与二极管激光联合使用对变形链球菌生存能力的抗菌作用。
将变形链球菌的细菌悬液分别暴露于0.1% TBO联合（20 mW，633 nm二极管激光，连续模式，150秒）或0.1%卟吩姆钠及激光照射（100 mW，662 nm二极管激光，连续模式，120秒）。对照组分别单独接受激光照射、单独使用TBO/卟吩姆钠或既不使用TBO/卟吩姆钠也不接受激光照射。然后将悬液铺在特定的琼脂平板上，于37℃有氧培养。最后，根据菌落形成评估杀菌效果。
仅在TBO光致敏和3 J/cm²激光照射后观察到潜在的细菌细胞杀伤（p<0.05），而卟吩姆钠在黑暗条件下与激光照射相比显示出显著降低（p<0.05）。
在体外显著降低变形链球菌的生存能力方面，TBO介导的光动力疗法似乎比卟吩姆钠更有效。
