使用拉达氯林（Radachlorin®）进行抗菌光动力疗法对金黄色葡萄球菌和大肠杆菌的影响：一项体外研究。
The effect of antimicrobial photodynamic therapy with radachlorin® on Staphylococcus aureus and Escherichia coli: an in vitro study.
作者信息
Fekrazad Reza, Zare Hadi, Mohammadi Sepahvand Sara, Morsali Parisa
机构信息
Department of Dentistry, AJA University of Medical Sciences, Tehran, Iran.
Periodontic Department, Faculty of Dentistry, Tehran University of Medical sciences, Tehran, Iran.
出版信息
J Lasers Med Sci. 2014 Spring;5(2):82-5.
INTRODUCTION
The aim of this study is the evaluation of the effect of Antimicrobial Photodynamic Therapy with Radachlorin on Staphylococcus aureus and Escherichia coli. New windows are open in the antimicrobial field so-call Photodynamic therapy that incorporates a nonpoisonous photosensitizer (PS) with innocuous special wavelength photons to excite the PS.
METHODS
Two strains of bacteria used in this study were Methicillin resistant Staphylococcus aureus (ATCC 33591; PTCC 1764) and Escherichia coli (ATCC 25922; PTCC1399). Concentrations of 0.2 ml of Radachlorin® were applied on 0.2 ml of bacterial suspensions and placed in a 48-well microtiter plate. The following groups were used: (I) L- PS- (no laser, no photosensitizer), (II) L-PS+ (treated only with PS), (III) L+ PS- (treated only with laser) and (IV) L+ PS+ (treated with laser and PS: photodynamic therapy group). Aliquots of bacterial suspensions were sensitized with Radachlorin® for 15 minutes in the dark at room temperature and then bacterial suspensions in group III and IV were irradiated with 210 mW (power density) and 12 J/cm2 (energy density) on continuous mode.
RESULTS
This study showed that photodynamic therapy reduces 0.14 log 10 in E.Coli (group IV) and there were significant differences for group IV (P<0.01). Photodynamic therapy in S.Aureus showed 6.28 log 10 colony count reduction (group IV) and there were highly significant differences in Photodynamic therapy group (P<0.0001).
CONCLUSION
Radachlorin® have bactericidal effect on S.aureus (6.28 log 10) and bacteriostaticeffect on E.coli (0.14 log 10).
引言
本研究旨在评估用拉达氯林进行抗菌光动力疗法对金黄色葡萄球菌和大肠杆菌的效果。抗菌领域开启了新的窗口，即所谓的光动力疗法，它将无毒的光敏剂（PS）与无害的特定波长光子相结合以激发光敏剂。
方法
本研究使用的两种细菌菌株分别是耐甲氧西林金黄色葡萄球菌（美国典型培养物保藏中心33591；伊朗国家菌种保藏中心1764）和大肠杆菌（美国典型培养物保藏中心25922；伊朗国家菌种保藏中心1399）。将0.2毫升的拉达氯林应用于0.2毫升的细菌悬液中，并置于48孔微量滴定板中。使用以下几组：（I）L - PS -（无激光，无光敏剂），（II）L - PS +（仅用光敏剂处理），（III）L + PS -（仅用激光处理）和（IV）L + PS +（用激光和光敏剂处理：光动力疗法组）。在室温黑暗条件下，将细菌悬液的等分试样用拉达氯林敏化15分钟，然后对III组和IV组的细菌悬液以连续模式用210毫瓦（功率密度）和12焦/平方厘米（能量密度）进行照射。
结果
本研究表明，光动力疗法使大肠杆菌（IV组）减少了0.14个对数10，IV组存在显著差异（P < 0.01）。金黄色葡萄球菌的光动力疗法显示菌落计数减少了6.28个对数1（IV组），光动力疗法组存在极显著差异（P < 0.0001）。
结论
拉达氯林对金黄色葡萄球菌有杀菌作用（6.28个对数10），对大肠杆菌有抑菌作用（0.14个对数10）。
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