Kim Ji-Won, Lim Hyun-Soo
Department of Biomedical Engineering, Graduate School, Chungnam National University, Daejeon 35015, Republic of Korea.
Department of Biomedical Engineering, Graduate School, Chungnam National University, Daejeon 35015, Republic of Korea; Department of Electric and Electronic Engineering, Faculty of Technology, Sakarya University of Applied Science, Esentepe Campus, Serdivan, Sakarya, Turkey.
Photodiagnosis Photodyn Ther. 2020 Sep;31:101931. doi: 10.1016/j.pdpdt.2020.101931. Epub 2020 Jul 24.
Pseudomonas aeruginosa is one of the most life-threatening pathogens in nosocomial environments. This study aimed to explore whether photodynamic therapy using a 660 nm diode laser and Radachlorin is an effective in vitro inhibitor of P. aeruginosa and determine what energy density and rate of delivery are the most impactful.
Suspensions ofP. aeruginosa were obtained, inoculated in petri dishes with Radachlorin, and incubated for 30 min. Then, the laser light of a 660 nm diode laser was irradiated at varying energy densities and rates of delivery. Cell viability was evaluated after culturing.
The combination of Radachlorin and the laser was more effective than Radachlorin or the laser alone (P < 0.05) in reducingP. aeruginosa and showed a killing rate of 95.8 % at an energy density of 5 J/cm. In addition, the low rate of photodynamic therapy delivery group was more effective in inhibiting P. aeruginosa compared to the high rate of photodynamic therapy delivery group (P < 0.05).
Within the limitations of thisin vitro study, we found that photodynamic therapy using a 660 nm diode laser and Radachlorin was effective for the inhibition of P. aeruginosa. In addition, energy density and rate of delivery are important factors to consider when using a 660 nm diode laser and Radachlorin to inhibit the growth of this organism.
铜绿假单胞菌是医院环境中最具生命威胁的病原体之一。本研究旨在探讨使用660nm二极管激光和二氢卟吩e6进行光动力疗法是否是一种有效的体外抑制铜绿假单胞菌的方法，并确定何种能量密度和照射速率最具影响力。
获取铜绿假单胞菌悬液，接种于含有二氢卟吩e6的培养皿中，孵育30分钟。然后，以不同的能量密度和照射速率照射660nm二极管激光。培养后评估细胞活力。
二氢卟吩e6与激光联合使用在减少铜绿假单胞菌方面比单独使用二氢卟吩e6或激光更有效（P<0.05），在能量密度为5J/cm时显示出95.8%的杀灭率。此外，与高光动力疗法照射速率组相比，低光动力疗法照射速率组在抑制铜绿假单胞菌方面更有效（P<0.05）。
在本体外研究的局限性内，我们发现使用660nm二极管激光和二氢卟吩e6进行光动力疗法对抑制铜绿假单胞菌有效。此外，在使用660nm二极管激光和二氢卟吩e6抑制该生物体生长时，能量密度和照射速率是需要考虑的重要因素。
