1.55μm 激发下近红外染料的双光子光学性质。
Two-photon optical properties of near-infrared dyes at 1.55 μm excitation.
机构信息
Department of Radiology, Washington University, St. Louis, Missouri 63110, USA.
出版信息
J Phys Chem B. 2011 Oct 6;115(39):11530-5. doi: 10.1021/jp207618e. Epub 2011 Sep 9.
Abstract
Two-photon (2P) optical properties of cyanine dyes were evaluated using a 2P fluorescence spectrophotometer with 1.55 μm excitation. We report the 2P characteristics of common NIR polymethine dyes, including their 2P action cross sections and the 2P excited fluorescence lifetime. One of the dyes, DTTC, showed the highest 2P action cross-section (∼103 ± 19 GM) and relatively high 2P excited fluorescence lifetime and can be used as a scaffold for the synthesis of 2P molecular imaging probes. The 2P action cross-section of DTTC and the lifetime were also highly sensitive to the solvent polarity, providing other additional parameters for its use in optical imaging and the mechanism for probing environmental factors. Overall, this study demonstrated the quantitative measurement of 2P properties of NIR dyes and established the foundation for designing molecular probes for 2P imaging applications in the NIR region.
摘要
使用配备 1.55μm 激发光的双光子（2P）荧光分光光度计评估了菁染料的 2P 光学性质。我们报告了常见近红外多甲川染料的 2P 特性，包括它们的 2P 作用截面和 2P 激发荧光寿命。其中一种染料 DTTC 显示出最高的 2P 作用截面（约 103±19 GM）和相对较高的 2P 激发荧光寿命，可作为合成 2P 分子成像探针的支架。DTTC 的 2P 作用截面和寿命也对溶剂极性非常敏感，为其在光学成像中的应用提供了其他附加参数，并为探测环境因素的机制提供了其他附加参数。总的来说，这项研究定量测量了近红外染料的 2P 性质，并为设计近红外区域的 2P 成像应用的分子探针奠定了基础。
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