Laboratory of Immunobiology for Research and Diagnosis Applications (LIRAD), Blood and Tissue Bank, Germans Trias i Pujol Research Institute, Carretera Canyet s/n, Badalona, Spain.
Transl Res. 2011 Sep;158(3):140-5. doi: 10.1016/j.trsl.2011.04.004. Epub 2011 May 30.
The regenerating gene (REG) is a multigene family in humans that plays a role in tissue regeneration. The REG Iα protein is expressed in the pancreas and the gastrointestinal tract and is involved in the pathophysiology of gastritis, pancreatitis, cancer, inflammatory bowel disease, and type 1 diabetes (T1D). Celiac disease (CD) is an autoimmune disease caused by the ingestion of gluten in genetically susceptible individuals. Our aim was to determine whether the serum REG Iα concentration reflects the destructive/regenerative process in the small bowel in CD. REG Iα was determined by enzyme-linked immunosorbent assay (ELISA) in 40 patients with active CD, and in 19 of them, REG Iα was assessed after following a gluten free diet. As controls, 35 healthy subjects were included in the study. Autoantibodies to transglutaminase, gliadin, and endomisium were measured also. We found a significant increase in REG Iα in the sera of CD patients when compared with controls. REG Iα levels decreased after a gluten-free diet together with a significant reduction in antitransglutaminase antibodies. T1D and pernicious anemia patients displayed normal serum REG Iα concentrations. This preliminary study suggests that REG Iα protein levels can be used as a biomarker for the diagnosis and monitoring of CD.
再生基因（REG）是人类中的一个多基因家族，在组织再生中发挥作用。REG Iα 蛋白在胰腺和胃肠道中表达，参与胃炎、胰腺炎、癌症、炎症性肠病和 1 型糖尿病（T1D）的病理生理学。乳糜泻（CD）是一种自身免疫性疾病，由遗传易感个体摄入麸质引起。我们的目的是确定血清 REG Iα 浓度是否反映了 CD 中小肠的破坏/再生过程。通过酶联免疫吸附试验（ELISA）在 40 例活动性 CD 患者中测定 REG Iα，并在其中 19 例患者中在遵循无麸质饮食后评估 REG Iα。作为对照，研究纳入了 35 名健康受试者。还测量了针对转谷氨酰胺酶、麦胶和内肌膜的自身抗体。与对照组相比，我们发现 CD 患者血清中的 REG Iα 显著增加。无麸质饮食后，REG Iα 水平下降，同时抗转谷氨酰胺酶抗体显著减少。T1D 和恶性贫血患者的血清 REG Iα 浓度正常。这项初步研究表明，REG Iα 蛋白水平可用作 CD 的诊断和监测的生物标志物。
