利用裂殖酵母粟酒裂殖酵母研究G2期DNA损伤检查点。
Studying G2 DNA damage checkpoints using the fission yeast Schizosaccharomyces pombe.
作者信息
Willis Nicholas, Rhind Nicholas
机构信息
Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA.
出版信息
Methods Mol Biol. 2011;782:1-12. doi: 10.1007/978-1-61779-273-1_1.
Abstract
Using synchronized cells, one can directly measure delay in mitosis brought about by the G2 DNA damage checkpoint in response to exposure to exogenous DNA damaging agents. Scoring mitosis in the fission yeast Schizosaccharomyces pombe is relatively simple. Many techniques exist for synchronizing cells for such assays. We present a detailed explanation of the setup and use of centrifugal elutriation to synchronize cells in G2, exposure of cells to DNA damage, and measurement of mitotic progression and delay.
摘要
使用同步化细胞，可以直接测量在暴露于外源性DNA损伤剂时，由G2期DNA损伤检查点引起的有丝分裂延迟。在裂殖酵母粟酒裂殖酵母中对有丝分裂进行评分相对简单。存在许多用于同步化细胞以进行此类测定的技术。我们详细解释了使用离心淘析法将细胞同步化至G2期、使细胞暴露于DNA损伤以及测量有丝分裂进程和延迟的设置与操作。
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