制备温敏和 pH 敏感聚合物磁性水凝胶纳米球作为抗癌药物载体。

Preparation of thermoresponsive and pH-sensitivity polymer magnetic hydrogel nanospheres as anticancer drug carriers.

机构信息

National Center for Nanoscience and Technology, Laboratory of Nanobiomedicine and Nanosafety, Division of Nanomedicine and Nanobiology, No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China.

出版信息

Colloids Surf B Biointerfaces. 2011 Dec 1;88(2):593-600. doi: 10.1016/j.colsurfb.2011.07.048. Epub 2011 Jul 30.

DOI:10.1016/j.colsurfb.2011.07.048

PMID:21871786

Abstract

In this work, a novel thermo and pH responsive magnetic hydrogel nanosphere poly(N-isopropylacrylamide-co-acrylic acid)/Fe(3)O(4) (poly(NIPAAm-co-AA)/Fe(3)O(4)) has been successfully prepared. The magnetic hydrogel nanospheres with thermo and pH-sensitivity were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), Fourier transform infrared-spectrometer (FT-IR), UV-vis absorption spectroscopy, and vibrating sample magnetometer (VSM). The magnetic hydrogel nanospheres exhibited uniform sphere structures and superparamagnetic property. Finally, the drug loading capacities and the releasing behavior of the magnetic hydrogel nanospheres were investigated with doxorubicin hydrochloride (DOX) as an anticancer drug model. The resulting magnetic hydrogel nanospheres exhibited high encapsulation efficiency (95%) to DOX under an appropriate condition. In vitro release experiments revealed that release was faster at pH 5.3 (37°C) than at pH 7.4 (25°C) or pH 7.4 (37°C). The DOX-loaded magnetic hydrogel nanospheres also showed enhanced anticancer effect compared with the free drug in vitro. These presented results suggested that the magnetic hydrogel nanospheres have a potential as tumor targeting drug carrier.

摘要

在这项工作中，成功制备了一种新型的温敏和 pH 响应磁性水凝胶纳米球聚（N-异丙基丙烯酰胺-co-丙烯酸）/Fe3O4（poly（NIPAAm-co-AA）/Fe3O4）。具有温敏和 pH 敏感性的磁性水凝胶纳米球通过透射电子显微镜（TEM）、动态光散射（DLS）、傅里叶变换红外光谱仪（FT-IR）、紫外-可见吸收光谱和振动样品磁强计（VSM）进行了表征。磁性水凝胶纳米球呈现出均匀的球形结构和超顺磁性。最后，以盐酸阿霉素（DOX）为抗癌药物模型，研究了磁性水凝胶纳米球的载药能力和释放行为。在适当的条件下，所得磁性水凝胶纳米球对 DOX 的包封率高达 95%。体外释放实验表明，在 pH 5.3（37°C）下的释放速度比在 pH 7.4（25°C）或 pH 7.4（37°C）下更快。载药磁性水凝胶纳米球在体外也显示出比游离药物更强的抗癌效果。这些结果表明，磁性水凝胶纳米球具有作为肿瘤靶向药物载体的潜力。