Zhang Zhuliang, Peng Minsi, Li Defeng, Yao Jun, Li Yingxue, Wu Benhua, Wang Lisheng, Xu Zhenglei
Department of Gastroenterology, Shenzhen People's Hospital, The Second Clinical Medical College, Jinan University, Shenzhen, China.
Front Chem. 2021 Aug 31;9:736271. doi: 10.3389/fchem.2021.736271. eCollection 2021.
Gastric cancer is one of the most common malignant tumors, and early diagnosis will be of great significance to improve the survival quality and overall treatment outcome evaluation of patients. Nanoelectrochemical immunosensor is an emerging biosensor combining nanotechnology, electrochemical analysis method and immunological technology, which has simple operation, fast analysis speed, high sensitivity, and good selectivity. This mini-review summarized immunoassay techniques, nanotechnology and electrochemical sensing for the early detection of gastric cancer. In particular, we focus on the tension of carbon nanomaterials in this field, including the functionalized preparation of materials, signal enhancement and the construction of novel sensing interfaces. Currently, various tumor markers are being developed, but the more recognized gastric cancer tumor markers are carcinoembryonic antigen (CEA), carbohydrate antigen (CA), CD44V9, miRNAs, and programmed death ligand 1. Among them, the electrochemical immunosensor allows the detection of CEA, CA, and miRNAs. The mini-review focused on the development of using carbon based materials, especially carbon nanotubes and graphene for immunosensor fabrication and gastric cancer markers detection.
胃癌是最常见的恶性肿瘤之一，早期诊断对于提高患者的生存质量和整体治疗效果评估具有重要意义。纳米电化学免疫传感器是一种新兴的生物传感器，它结合了纳米技术、电化学分析方法和免疫技术，具有操作简单、分析速度快、灵敏度高和选择性好等优点。本综述总结了用于胃癌早期检测的免疫分析技术、纳米技术和电化学传感。特别地，我们关注了该领域中碳纳米材料的应用，包括材料的功能化制备、信号增强以及新型传感界面的构建。目前，各种肿瘤标志物正在被研发，但较为公认的胃癌肿瘤标志物有癌胚抗原（CEA）、糖类抗原（CA）、CD44V9、微小RNA（miRNAs）和程序性死亡配体1。其中，电化学免疫传感器可用于检测CEA、CA和miRNAs。本综述重点介绍了使用碳基材料，特别是碳纳米管和石墨烯制备免疫传感器以及检测胃癌标志物的进展。
