Guo Yang, Feng Liang
Department of Breast Surgery, The First Affiliated Hospital of China Medical University, Shenyang, China.
Front Chem. 2022 May 12;10:898924. doi: 10.3389/fchem.2022.898924. eCollection 2022.
Tumor markers are one of the important indicators for early cancer diagnosis. As a new analytical method, electrochemical immunosensing analysis has the advantages of high sensitivity, good selectivity, and rapid detection, which is of great significance for the detection of tumor markers. In this work, an AuNP/reduced graphene oxide (AuNP/rGO) composite was synthesized. We used it for electrochemical sensor fabrication with the assistance of the biotin-streptavidin protein (SA) system to further amplify the signal to achieve sensitive detection of carcinoembryonic antigen (CEA). In addition, AuNPs have been incorporated due to their good electrical conductivity and biocompatibility, which can accelerate electron transfer at the electrode interface and improve the loading capacity to capture antibodies. The fabricated AuNPs/SA/rGO has a large working surface area and high material utilization ratio, which improves the catalytic capacity of HO reduction and effectively amplifies the current signal. The linear range of the response current signal of the sensor toward the CEA concentration is 20 fg/ml to 200 ng/ml, and the limit of detection can achieve 6.2 fg/ml. In addition, the fabricated immunosensor has good reproducibility, selectivity, and stability.
肿瘤标志物是早期癌症诊断的重要指标之一。作为一种新型分析方法，电化学免疫传感分析具有灵敏度高、选择性好、检测快速等优点，对肿瘤标志物的检测具有重要意义。在本工作中，合成了金纳米粒子/还原氧化石墨烯（AuNP/rGO）复合材料。我们借助生物素-链霉亲和素蛋白（SA）系统将其用于电化学传感器的制备，以进一步放大信号，实现对癌胚抗原（CEA）的灵敏检测。此外，由于金纳米粒子具有良好的导电性和生物相容性，已将其引入，这可以加速电极界面的电子转移并提高捕获抗体的负载能力。所制备的AuNPs/SA/rGO具有较大的工作表面积和较高的材料利用率，提高了对HO还原的催化能力并有效放大了电流信号。该传感器对CEA浓度的响应电流信号线性范围为20 fg/ml至200 ng/ml，检测限可达6.2 fg/ml。此外，所制备的免疫传感器具有良好的重现性、选择性和稳定性。
