检测来自猪场粪便处理系统的水样中的圆环病毒和猪腺病毒。
Detection of circoviruses and porcine adenoviruses in water samples collected from swine manure treatment systems.
机构信息
Departamento de Microbiologia e Parasitologia, Laboratório de Virologia Aplicada, Universidade Federal de Santa Catarina, 88040-900 Florianópolis, Santa Catarina, Brazil.
出版信息
Res Vet Sci. 2012 Aug;93(1):538-43. doi: 10.1016/j.rvsc.2011.07.022. Epub 2011 Aug 27.
Samples collected from two swine manure treatment systems including: swine manure treatment system and demonstrative unit (SMTS and DU), were analyzed by qPCR to quantify the amount of porcine adenovirus (PAdV) and porcine circovirus (PCV2) present. Positive samples were tested for virus integrity using DNase assay. Fifty-six water samples were collected monthly from March 2009 to May 2010. PAdV genome was found 66% of the samples in the SMTS and in 78% of the samples in the DU system. PCV2 was detected in 96% of samples collected from the SMTS system and in 86% of samples from DU. DNase assay revealed that there were undamaged virus particles of both PAdV and PCV2 in all sampling sites in the SMTS. However, undamaged particles of both viruses were detected in samples from the DU system in the affluent and middle sites, though undamaged PCV2 was absent in the effluent samples.
从两个猪粪处理系统（猪粪处理系统和示范单元）中采集样本，通过 qPCR 进行分析，以定量存在的猪腺病毒（PAdV）和猪圆环病毒（PCV2）数量。使用 DNA 酶检测对阳性样本进行病毒完整性测试。从 2009 年 3 月至 2010 年 5 月，每月采集 56 份水样。在 SMTS 中，PAdV 基因组在 66%的样本中被发现，在 DU 系统中在 78%的样本中被发现。从 SMTS 系统采集的 96%的样本中检测到了 PCV2，而 DU 系统的 86%的样本中检测到了 PCV2。DNA 酶检测显示，在 SMTS 的所有采样点均存在 PAdV 和 PCV2 的完整病毒颗粒。然而，在 DU 系统的富中和中等采样点中检测到了两种病毒的完整颗粒，但在流出物样本中未检测到完整的 PCV2。
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