Dos Santos Debora Regina Lopes, Silva-Sales Marcelle, Fumian Tulio Machado, Maranhão Adriana Gonçalves, Malta Fábio Correia, Ferreira Fernando César, Pimenta Marcia Maria, Miagostovich Marize Pereira
Department of Veterinary Microbiology and Immunology, Universidade Federal Rural Do Rio de Janeiro, Seropédica, Rio de Janeiro, Brazil.
Institute of Public Health and Tropical Pathology, Universidade Federal de Goiás, Goiânia, Goiás, Brazil.
Food Environ Virol. 2023 Mar;15(1):21-31. doi: 10.1007/s12560-022-09544-x. Epub 2023 Jan 11.
This study assessed the sources of contamination of water matrices in a rural area using detection of a host-specific virus (human adenovirus [HAdV], porcine adenovirus [PAdV] and bovine polyomaviruses [BoPyV]) as potential microbial source-tracking tool, and rotavirus A [RVA], given its epidemiological importance in Brazil. From July 2017 to June 2018, 92 samples were collected from eight points (P1-P8) of surface and raw waters in southeastern region of Brazil. Fifty-five (59.8%) were positive for HAdV, 41 (44.5%) for RVA, 10 (10.9%) for PAdV and four (4.3%) for BoPyV. HAdV and RVA were detected at all sites, and over the entire sampling period, PAdV was detected at a porcine breeding area and at Guarda River site, presenting high concentrations up to 2.6 × 10 genome copies per liter [GC/L], and viral concentrations ranging from 9.6 × 10 to 7.1 × 10, while BoPyV (1.5 × 10 GC/L-9.2 × 10 GC/L) was only detected in samples from the bovine breeding areas. The combination of human and animal virus circulation presents a potential impact in the environment due to raw sewage discharge from regional communities, as well as potential hazard to human and animal health.
本研究通过检测宿主特异性病毒（人类腺病毒[HAdV]、猪腺病毒[PAdV]和牛多瘤病毒[BoPyV]）作为潜在的微生物源追踪工具，并鉴于轮状病毒A[RVA]在巴西的流行病学重要性，评估了农村地区水基质的污染来源。2017年7月至2018年6月，从巴西东南部地区的地表水和原水的八个采样点（P1 - P8）采集了92个样本。55个（59.8%）样本HAdV呈阳性，41个（44.5%）样本RVA呈阳性，10个（10.9%）样本PAdV呈阳性，4个（4.3%）样本BoPyV呈阳性。在所有采样点均检测到HAdV和RVA，在整个采样期间，在一个养猪场区域和瓜尔达河采样点检测到PAdV，其浓度高达每升2.6×10基因组拷贝数[GC/L]，病毒浓度范围为9.6×10至7.1×10，而BoPyV（1.5×10 GC/L - 9.2×10 GC/L）仅在养牛场区域的样本中检测到。人类和动物病毒的共同传播对环境有潜在影响，原因是区域社区排放未经处理的污水，同时对人类和动物健康也存在潜在危害。
