Creasey A, Fenton B, Walker A, Thaithong S, Oliveira S, Mutambu S, Walliker D
University of Edinburgh, Scotland, United Kingdom.
Am J Trop Med Hyg. 1990 May;42(5):403-13. doi: 10.4269/ajtmh.1990.42.403.
Sixty Plasmodium falciparum isolates, 20 each from Thailand, Zimbabwe, and Brazil, were characterized for 20 variant genetic markers, including the enzymes glucose phosphate isomerase, adenosine deaminase and peptidase, 11 other proteins detected by 2-dimensional electrophoresis (2D-PAGE), 2 merozoite surface antigens (MSA-1 and MSA-2), one exported antigen (Exp-1), and sensitivity to the drugs chloroquine, pyrimethamine, and mefloquine. The study examines the extent of diversity between individual isolates and the differences in the frequency of certain variants of the markers between the 3 countries. The principal conclusions to be drawn from the study are that there is extensive polymorphism in many of the genetically determined characters of this parasite, multiple infections with greater than 1 genetically distinct parasite are common, and there are geographical variations in the frequencies with which variant forms of certain markers occur.
对来自泰国、津巴布韦和巴西的60株恶性疟原虫分离株（每个国家各20株）进行了20种变异遗传标记的特征分析，这些标记包括磷酸葡萄糖异构酶、腺苷脱氨酶和肽酶等酶类、通过二维电泳（2D-PAGE）检测到的其他11种蛋白质、两种裂殖子表面抗原（MSA-1和MSA-2）、一种输出抗原（Exp-1）以及对氯喹、乙胺嘧啶和甲氟喹的敏感性。该研究考察了各个分离株之间的多样性程度以及这3个国家之间某些标记变异体频率的差异。该研究得出的主要结论是，这种寄生虫的许多遗传决定特征存在广泛的多态性，感染有大于1种遗传上不同寄生虫的多重感染很常见，并且某些标记的变异形式出现的频率存在地理差异。
