Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Korea.
J Microbiol Biotechnol. 2011 Aug;21(8):846-53. doi: 10.4014/jmb.1103.03009.
Glycerol has become an attractive carbon source in the biotechnology industry owing to its low price and reduced state. However, glycerol is rarely used as a carbon source in Saccharomyces cerevisiae because of its low utilization rate. In this study, we used glycerol as a main carbon source in S. cerevisiae to produce 1,2-propanediol. Metabolically engineered S. cerevisiae strains with overexpression of glycerol dissimilation pathway genes, including glycerol kinase (GUT1), glycerol 3-phosphate dehydrogenase (GUT2), glycerol dehydrogenase (gdh), and a glycerol transporter gene (GUP1), showed increased glycerol utilization and growth rate. More significant improvement of glycerol utilization and growth rate was accomplished by introducing 1,2-propanediol pathway genes, mgs (methylglyoxal synthase) and gldA (glycerol dehydrogenase) from Escherichia coli. By engineering both glycerol dissimilation and 1,2-propanediol pathways, the glycerol utilization and growth rate were improved 141% and 77%, respectively, and a 2.19 g 1,2- propanediol/l titer was achieved in 1% (v/v) glycerolcontaining YEPD medium in engineered S. cerevisiae.
由于价格低廉且处于还原态，甘油已成为生物技术行业中颇具吸引力的碳源。然而，由于其利用率低，甘油很少被用作酿酒酵母中的碳源。在这项研究中，我们使用甘油作为酿酒酵母的主要碳源来生产 1,2-丙二醇。通过过表达甘油分解途径基因，包括甘油激酶（GUT1）、甘油 3-磷酸脱氢酶（GUT2）、甘油脱氢酶（gdh）和甘油转运蛋白基因（GUP1），使代谢工程化的酿酒酵母菌株能够利用甘油并提高生长速率。通过引入大肠杆菌中的 1,2-丙二醇途径基因 mgs（甲基乙二醛合酶）和 gldA（甘油脱氢酶），可以显著提高甘油的利用率和生长速率。通过工程化甘油分解和 1,2-丙二醇途径，甘油的利用率和生长速率分别提高了 141%和 77%，在含有 1%（v/v）甘油的 YEPD 培养基中，工程化酿酒酵母的 1,2-丙二醇产量达到 2.19 g/L。
