伊朗伊斯法罕地区从屠宰场到零售阶段的农场动物中艰难梭菌的分离与鉴定
Isolation and characterization of Clostridium difficile in farm animals from slaughterhouse to retail stage in Isfahan, Iran.
作者信息
Esfandiari Zahra, Weese J Scott, Ezzatpanah Hamid, Chamani Mohammad, Shoaei Parisa, Yaran Majid, Ataei Behrooz, Maracy Mohammad Reza, Ansariyan Akbar, Ebrahimi Fatemeh, Jalali Mohammad
机构信息
1 Department of Research and Development, Deputy of Food and Drug, Isfahan University of Medical Sciences , Isfahan, Iran .
2 Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences , Isfahan, Iran .
出版信息
Foodborne Pathog Dis. 2015 Oct;12(10):864-6. doi: 10.1089/fpd.2014.1910.
To determine the prevalence of Clostridium difficile in farm animals from slaughterhouse through to retail stage, a total of 750 samples of feces, posteviscerated and washed carcass were collected from cattle, camels, goats, and sheep in Isfahan, Iran. The overall prevalence of C. difficile in feces, posteviscerated and washed carcass were 20 (13.3%), 23 (15.3%), and 11 (7.3%), respectively; while C. difficile was isolated from 79 (26.3%) retail samples. Twenty-nine (3.8%) isolates were toxigenic, with most toxigenic isolates (n = 17, 5.6%) identified from the retail stage. All toxigenic isolates harbored tcdA and tcdB; however, all were negative for cdtB. The 29 isolates were classified into 21 different ribotypes. This study revealed evidence of existence of toxigenic C. difficile in farm animal feces and meat in Iran.
为确定从屠宰场到零售阶段的农场动物中艰难梭菌的流行情况，从伊朗伊斯法罕的牛、骆驼、山羊和绵羊中总共采集了750份粪便、去内脏并洗净的胴体样本。粪便、去内脏并洗净的胴体中艰难梭菌的总体流行率分别为20（13.3%）、23（15.3%）和11（7.3%）；而从79份（26.3%）零售样本中分离出了艰难梭菌。29株（3.8%）分离株具有产毒能力，大多数产毒分离株（n = 17，5.6%）是在零售阶段鉴定出来的。所有产毒分离株都携带tcdA和tcdB；然而，所有分离株的cdtB均为阴性。这29株分离株被分为21种不同的核糖体分型。本研究揭示了伊朗农场动物粪便和肉类中存在产毒艰难梭菌的证据。
Zotero 插件