Department of Integrated Traditional Chinese and Western Medicine, Zhongnan Hospital, Wuhan University, Wuhan, Hubei, China.
Nat Prod Res. 2012;26(11):1022-6. doi: 10.1080/14786419.2010.546796. Epub 2011 Aug 31.
Diabetic nephropathy (DN) is the leading cause of end-stage failure of the kidney, but the efficacy of currently available strategies for the prevention of DN remains unsatisfactory. In this study, we investigated the effects of Danggui Buxue Tang (DBT), a Chinese herbal decoction prepared from Radix Astragali (RA) and Radix Angelicae sinensis (RAS), on high glucose-induced proliferation and expression of laminin, type IV collagen (collagen IV) and fibronectin in glomerular mesangial cells (GMCs). The cell proliferation was determined by MTT assay, and the expression of collagen IV, laminin and fibronectin in GMCs was detected by ELISA assay. It was shown that high glucose clearly induced the proliferation of GMCs and increased the release of collagen IV, laminin and fibronectin. Treatment with RA, RAS and DBT inhibited cell proliferation and the expression of collagen IV, laminin and fibronectin induced by high glucose, with DBT, especially at the highest concentration (DBT20), exhibiting a stronger effect than RA and RAS alone. Thus, it is concluded that DBT inhibits increased cell proliferation and the expression of major extracellular matrix proteins that are induced by high glucose, indicating its value for prophylaxis and therapy of DN at the early stages.
糖尿病肾病 (DN) 是导致肾脏终末期衰竭的主要原因，但目前用于预防 DN 的策略的疗效仍不尽人意。在这项研究中，我们研究了当归补血汤（DBT）对高糖诱导的肾小球系膜细胞（GMC）增殖和层粘连蛋白、IV 型胶原（胶原 IV）和纤维连接蛋白表达的影响。DBT 是由黄芪（RA）和当归（RAS）组成的中药方剂。通过 MTT 测定法测定细胞增殖，通过 ELISA 测定法检测 GMC 中胶原 IV、层粘连蛋白和纤维连接蛋白的表达。结果表明，高葡萄糖明显诱导 GMC 增殖，并增加胶原 IV、层粘连蛋白和纤维连接蛋白的释放。RA、RAS 和 DBT 治疗可抑制高葡萄糖诱导的细胞增殖和胶原 IV、层粘连蛋白和纤维连接蛋白的表达，其中 DBT（尤其是在最高浓度 DBT20）的作用强于单独使用 RA 和 RAS。因此，结论是 DBT 抑制了高葡萄糖诱导的细胞增殖和主要细胞外基质蛋白的表达，表明其在早期预防和治疗 DN 方面具有价值。
