没食子酸鸡纳树提取物的急性毒性和肝毒性动力学研究。
Acute toxicity and hepatotoxicokinetic studies of Tamarindus indica extract.
机构信息
Applied and Environmental Microbiology Research Group, Department of Biochemistry and Microbiology, University of Fort Hare, Alice, South Africa.
出版信息
Molecules. 2011 Aug 31;16(9):7415-27. doi: 10.3390/molecules16097415.
Tamarindus indica is widely used as a food and beverage and in traditional medicine. The apparent lack of dose standardization in herbal medicine necessitates the evaluation of the lethality T. indica on Artemia salina nauplii and chicken embryos via in vitro and in vivo techniques. Furthermore, hepatotoxicokinetics of the crude extract and fractions on Wister rats was also assessed. At concentrations of 200, 20 and 2 µg/mL, crude extract and fractions showed brine shrimp death percentages ranging from 86.70% to 3.30% and the sub-fractions showed death percentage ranges of 46.70% to 3.30%. Calculated LD₅₀ values ranged from 832 µg/mL to 5,019 µg/mL. Dosing Wister rats with 25% and 50% concentration of LD₅₀ determined for crude extract and fractions on chicken embryos showed an elevation in the ALT and AST levels in the serum. Brine shrimps and chicken embryos showed a positive correlation, with R² values of 0.541 and 0.588 (P ≤ 0.05) for fractions and subfractions, respectively, as media for the lethality assay. Dose standardization in folk herbal medicine is imperative as T. indica used as food and medicine has been shown to be toxic at high doses. Brine shrimp and chicken embryos may be comparably used as medium for toxicity assay.
罗望子被广泛用作食品和饮料以及传统医学。草药中明显缺乏剂量标准化，因此需要通过体外和体内技术评估罗望子对卤虫无节幼体和鸡胚的致死性。此外，还评估了粗提取物和馏分对 Wister 大鼠的肝毒性动力学。在 200、20 和 2 µg/mL 浓度下，粗提取物和馏分显示卤虫死亡率范围为 86.70%至 3.30%，亚馏分显示死亡率范围为 46.70%至 3.30%。计算的 LD₅₀ 值范围为 832 µg/mL 至 5019 µg/mL。用鸡胚中确定的粗提物和馏分的 LD₅₀ 的 25%和 50%浓度给 Wister 大鼠给药，显示血清中转氨酶（ALT 和 AST）水平升高。卤虫和鸡胚显示出正相关，馏分和亚馏分的 R² 值分别为 0.541 和 0.588（P ≤ 0.05），作为致死性测定的介质。由于高剂量的罗望子作为食物和药物已被证明具有毒性，因此在民间草药中进行剂量标准化是必要的。卤虫和鸡胚可作为毒性测定的类似介质使用。
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