Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520-8104, USA.
Mol Cell. 2011 Sep 2;43(5):703-12. doi: 10.1016/j.molcel.2011.07.013.
The COP10-DET1-DDB1 (CDD) complex is an evolutionarily conserved protein complex discovered for its role in the repression of photomorphogenesis in Arabidopsis. It is important in many cellular and developmental processes in both plants and animals, but its molecular mode of action remains poorly understood. Here, we show that the CDD component DET1 possesses transcriptional repression activity and physically interacts with two closely related MYB transcription factors, CCA1 and LHY, which are core components of the plant circadian clock. DET1 associates with the promoter of CCA1/LHY target genes, such as TOC1, in a CCA1/LHY-dependent manner and is required for their repression, suggesting a recruitment of DET1 by the central oscillator components to regulate the clock. Our results reveal DET1 as a core transcriptional repression factor important for clock progression. Overall, the CDD complex may function as a transcriptional corepressor in diverse processes through direct interaction with distinct transcription factors.
COP10-DET1-DDB1 (CDD) 复合物是一种进化上保守的蛋白质复合物，因其在拟南芥光形态建成中的抑制作用而被发现。它在植物和动物的许多细胞和发育过程中都很重要，但它的分子作用模式仍知之甚少。在这里，我们表明 CDD 成分 DET1 具有转录抑制活性，并与两个密切相关的 MYB 转录因子 CCA1 和 LHY 相互作用，CCA1 和 LHY 是植物生物钟的核心成分。DET1 以 CCA1/LHY 依赖的方式与 CCA1/LHY 靶基因如 TOC1 的启动子结合，并需要其抑制，这表明 DET1 被中央振荡器组件招募来调节时钟。我们的结果揭示了 DET1 作为一个核心转录抑制因子，对时钟进展很重要。总的来说，CDD 复合物可能通过与不同的转录因子直接相互作用，作为一种转录核心抑制剂在多种过程中发挥作用。
