Institute of Molecular Life Sciences, University of Zurich, Winterthurerstrasse 190, Zurich CH-8057, Switzerland.
EMBO Rep. 2011 Oct 28;12(11):1144-52. doi: 10.1038/embor.2011.165.
Wnt proteins are secreted, lipid-modified glycoproteins that control animal development and adult tissue homeostasis. Secretion of Wnt proteins is at least partly regulated by a dedicated machinery. Here, we report a genome-wide RNA interference screen for genes involved in the secretion of Wingless (Wg), a Drosophila Wnt. We identify three new genes required for Wg secretion. Of these, Emp24 and Eclair are required for proper export of Wg from the endoplasmic reticulum (ER). We propose that Emp24 and Eca act as specific cargo receptors for Wg to concentrate it in forming vesicles at sites of ER export.
Wnt 蛋白是一种分泌型的脂修饰糖蛋白，它可以控制动物的发育和成年组织的稳态。Wnt 蛋白的分泌至少部分受到专门的机制调控。在这里，我们报道了一个针对参与 Wingless（Wg）分泌的基因的全基因组 RNA 干扰筛选，Wg 是一种果蝇 Wnt。我们鉴定了三个新的基因，它们是 Wg 分泌所必需的。其中，Emp24 和 Eclair 对于 Wg 从内质网（ER）的正确输出是必需的。我们提出，Emp24 和 Eca 作为 Wg 的特异性货物受体，将其集中在 ER 输出部位形成囊泡。
