Department of Materials, Imperial College London, Prince Consort Road, London SW72AZ, United Kingdom.
J Biomed Mater Res A. 2011 Dec 1;99(3):435-44. doi: 10.1002/jbm.a.33185. Epub 2011 Sep 1.
Highly porous (> 90% porosity) 45S5 Bioglass®-derived glass-ceramic scaffolds were fabricated by foam replication method, and coated with carbon nanotubes (CNT) (coating thickness: 1 μm) using electrophoretic deposition (EPD). In vitro cell culture using mesenchymal stem cells (MSCs) was carried out on both scaffold systems (with and without CNT coating) over a 4-week period. By using AlamarBlue™, BSA and alkaline phosphatase assays; the cell viability and differentiation were measured quantitatively measured and compared between the two scaffold types. The results showed that both scaffold systems are biocompatible with MSCs and they can support the cellular activity. No cytotoxic effects of CNT were observed under the conditions of the present experiments. Although a lower initial cell viability on the CNT-coated scaffolds was observed, no significant differences were found after 4 weeks of culture compared with the uncoated scaffolds. This work therefore shows that there is in principle no significant improvement of cellular responses by creating a CNT-coating on this type of highly bioactive scaffolds. However, the electrical conductivity introduced by the coating might have the potential to increase cell viability and differentiation when cell culture is carried out under the effect of electrical stimulation.
采用泡沫复制法制备了孔隙率大于 90%（高多孔）的 45S5 型生物玻璃衍生的玻璃陶瓷支架，并通过电泳沉积（EPD）方法在支架上涂覆了一层 1μm 厚的碳纳米管（CNT）。在体外细胞培养中，使用间充质干细胞（MSCs）在这两种支架系统（有和没有 CNT 涂层）上进行了为期 4 周的培养。通过使用 AlamarBlueTM、BSA 和碱性磷酸酶测定法，定量测量并比较了两种支架类型之间的细胞活力和分化情况。结果表明，两种支架系统均与 MSCs 生物相容，可支持细胞活性。在本实验条件下，未观察到 CNT 的细胞毒性作用。尽管在 CNT 涂层支架上观察到初始细胞活力较低，但与未涂层支架相比，培养 4 周后无显著差异。因此，这项工作表明，在这种高生物活性支架上涂覆 CNT 并没有从根本上显著改善细胞反应。然而，当在电刺激的作用下进行细胞培养时，涂层引入的导电性可能具有提高细胞活力和分化的潜力。
