Department of Engineering Enzo Ferrari, University of Modena and Reggio Emilia, Strada Vignolese 905/A, 41125 Modena, Italy.
Mater Sci Eng C Mater Biol Appl. 2013 Oct;33(7):3592-600. doi: 10.1016/j.msec.2013.04.028. Epub 2013 Apr 21.
Highly porous 45S5 Bioglass®-based scaffolds fabricated by a foam replication technique were coated with electrically conductive organic-inorganic hybrid layers containing graphene by a solution method. α,ω-Triethoxysilane terminated poly (ethylene glycol) and tetraethoxysilane were used as the precursors of the organic-inorganic hybrid coatings, that contained 1.5 wt.% of homogeneously dispersed graphene nanoplatelets. The resulting coated scaffolds retained their original high porosity and interconnected pore structure after coating. The presence of graphene did not impair the bioactivity of the scaffolds in simulated body fluid. Initial tests carried out using MG-63 cells demonstrated that both uncoated scaffolds and scaffolds coated with organic/inorganic hybrids containing graphene offered the cultured cells an adequate surface for cell attachment, spreading and expression of extracellular matrix. The results showed that scaffolds coated with graphene are biocompatible and they can support cellular activity. The electrical conductivity introduced by the coating might have the potential to increase tissue growth when cell culture is carried out under an applied electric field.
采用泡沫复制技术制备的高多孔 45S5 Bioglass®基支架，通过溶液法涂覆含有石墨烯的导电有机-无机杂化层。α，ω-三乙氧基硅烷封端的聚乙二醇和四乙氧基硅烷用作有机-无机杂化涂层的前体，其中含有均匀分散的石墨烯纳米片 1.5wt%。涂层后的支架保留了其原始的高孔隙率和相互连通的孔结构。石墨烯的存在并没有损害支架在模拟体液中的生物活性。使用 MG-63 细胞进行的初步测试表明，未经涂层的支架和涂覆有含有石墨烯的有机/无机杂化层的支架都为培养细胞提供了足够的表面，用于细胞附着、扩散和细胞外基质的表达。结果表明，涂覆有石墨烯的支架具有生物相容性，并且可以支持细胞活性。当在施加电场下进行细胞培养时，涂层引入的导电性可能具有促进组织生长的潜力。
